miR-34a-5p在结肠癌患者的表达及其与临床特征及预后的相关性研究

Expression of miR-34a-5p in colon cancer patients and its correlation with clinical characteristics and prognosis

目的探讨miR-34a-5p在结肠癌患者的表达水平,并分析其与结肠癌患者临床特征及预后的相关性。方法采用前瞻性队列研究的方法,选择2021年1月1日至2021年12月31日在新疆医科大学附属中医医院接受治疗的结肠癌伴腹膜转移患者,计划入样本60例,收集患者的癌灶组织和癌旁组织标本,实时定量PCR检测癌灶组织和癌旁组织标本miR-34a-5p相对表达量,分析miR-34a-5p相对表达量与结肠癌患者临床特征的相关性,对结肠癌患者进行随访,绘制Kaplan-Meier曲线并采用COX回归分析预后的相关因素。结果最终纳入结肠癌患者58例,癌灶组织miR-34a-5p相对表达量明显低于癌旁组织,差异有统计学意义(P<0.001)。以结肠癌患者癌灶组织miR-34a-5p相对表达量平均值分组,结果显示N分期、分化程度与miR-34a-5p表达水平相关。绘制Kaplan-Meier生存曲线,结果显示结肠癌miR-34a-5p低表达组生存率明显低于高表达组,差异有统计学意义(P=0.028)。单因素分析显示,T分期、N分期、分化程度、miR-34a-5p表达水平与结肠癌患者生存期有关,差异有统计学意义(P<0.05)。多因素COX回归模型结果提示,分化程度、miR-34a-5p表达水平是结肠癌患者预后生存期的独立影响因素。结论在结肠癌患者病灶组织中,miR-34a-5p表达水平降低,miR-34a-5p表达水平与N分期、分化程度相关。miR-34a-5p表达水平是预后生存期的独立影响因素。

Objective To investigate the expression level of miR-34a-5p in patients with colon cancer and analyze its correlation with the clinical characteristics and prognosis of these patients.Methods A prospective cohort study was conducted.Patients with colon cancer accompanied by peritoneal metastasis who underwent treatment in Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital from January 1,2021 to December 31,2021 were selected.A sample of 60 patients was planned,and the cancer focus and adjacent tissue samples of the patients were collected.Real-time quantitative PCR was used to detect the relative expression level of miR-34a-5p in the cancer focus and adjacent tissue samples,and the correlation between the relative expression level of miR-34a-5p and the clinical characteristics of colon cancer patients was analyzed.The patients with colon cancer were followed up,Kaplan-Meier curves were drawn,and prognostic factors were analyzed using COX regression.Results In the end,58 patients with colon cancer were included,and the relative expression level of miR-34a-5p in cancer focus tissues was significantly lower than that in adjacent tissues,with a statistically significant difference(P<0.001).Grouped by the average relative expression level of miR-34a-5p in cancer focus tissues of colon cancer patients,the results showed that N staging and differentiation degree were related to the expression level of miR-34a-5p.Drawing Kaplan-Meier survival curves,the results showed that the survival rate of the low expression group of miR-34a-5p in colon cancer was significantly lower than that of the high expression group,with a statistically significant difference(P=0.028).Univariate analysis showed that T staging,N staging,differentiation degree,and miR-34a-5p expression level were related to the survival period of colon cancer patients,with statistically significant differences(P<0.05).Multivariate COX regression model results suggested that differentiation degree and miR-34a-5p expression level were independent factors affecting the prognosis and survival period of colon cancer patients.Conclusion In the lesion tissues of colon cancer patients,the expression level of miR-34a-5p decreases,and the expression level of miR-34a-5p is related to N staging and differentiation degree.The expression level of miR-34a-5p is an independent factor affecting prognosis and survival period.