复方香鱼口服液不同组分的解热机制研究

Study on the antipyretic mechanism of different components of compound Xiangyu oral liquid

目的研究复方香鱼口服液不同组分对干酵母致热大鼠的解热作用及相关机制,探讨复方香鱼口服液解热作用的药效物质基础-药效-作用机制的内在关系。方法采用背部皮下注射干酵母法复制SD大鼠发热模型,测定给药后6 h内各组大鼠的肛温,计算体温上升的最大值(ΔT_(max))及体温反应指数(TRI),酶联免疫吸附测定法(ELISA)检测血浆中环磷酸腺苷(c AMP)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)及下丘脑c AMP、前列腺素E_(2)(PGE_(2))表达水平。结果与模型组比较,复方香鱼口服液全成分组、水提物成分组及醇提物成分组均能显著降低干酵母致热大鼠体温(P<0.01或P<0.05);复方香鱼口服液全成分组、水提物成分组、醇提物成分组大鼠血浆中c AMP、IL-6含量显著降低(P<0.01或P<0.05);复方香鱼口服液全成分组、醇提物成分组大鼠血浆中TNF-α含量显著降低(P<0.01);复方香鱼口服液全成分组、水提物成分组、醇提物成分组下丘脑中c AMP含量显著降低(P<0.01或P<0.05);复方香鱼口服液全成分组下丘脑中的PGE_(2)含量显著降低(P<0.05)。结论复方香鱼口服液水提物成分和醇提物成分具有良好的解热作用,水提物成分的解热作用机制可能与降低血浆中c AMP、IL-6和下丘脑c AMP水平有关;醇提物成分的解热作用可能与降低血浆中c AMP、TNF-α、IL-6和下丘脑c AMP水平有关。

Objective To investigate the antipyretic effect and mechanism of different components of compound Xiangyu oral liquid on dry yeast-induced fever in rats,and explore the internal relationship between the pharmacodynamic material basis,efficacy and mechanism of the antipyretic effect of components of compound Xiangyu oral liquid.Methods The fever model in SD rats was established by subcutaneous injection of dry yeast on the back.The anal temperature of rats was measured in each group in 6 hours after drug interventions.The maximum temperature rise(ΔT_(max))and temperature response index(TRI)were calculated.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of cyclic adenosine monophosphate(cAMP),TNF-α,IL-6 in plasma and cAMP and prostaglandin E_(2)(PGE_(2))in the hypothalamus.Results Compared with the model group,the whole components group,water extract group and ethanol extract group of compound Xiangyu oral liquid significantly reduced the body temperature of rats induced by dry yeast(P<0.01 or P<0.05).The content of cAMP and IL-6 in plasma of rats in whole components group,water extract component group and alcohol extract component group of compound Xiangyu oral liquid decreased significantly(P<0.01 or P<0.05).The content of TNF-αin plasma of rats in whole components group and alcohol extract component group decreased significantly(P<0.01).The cAMP content in hypothalamus of whole components group,water extract component group and alcohol extract component group was significantly decreased(P<0.01 or P<0.05).The content of PGE_(2)in hypothalamus of compound Xiangyu oral liquid group was clearly decreased(P<0.05).Conclusion The water extract and alcohol extract of compound Xiangyu oral liquid have good antipyretic effect.The antipyretic mechanism of water extract may be related to the decrease of plasma cAMP,IL-6 and hypothalamic cAMP levels.The antipyretic effect of the alcohol extract components may be related to the reduction of cAMP,TNF-α,IL-6 in plasma and cAMP levels in hypothalamus.