基于混料设计优化红毛藻中类胡萝卜素的提取工艺

Optimization of extraction process of carotenoids from Bangia fusco-purpurea based on mixture design

红毛藻是类胡萝卜素的天然来源之一,该研究以红毛藻为原料,旨在确定其类胡萝卜素提取的最佳条件。通过混料设计建立提取试剂组成比例与类胡萝卜素提取量之间的回归模型,得到红毛藻中类胡萝卜素提取的最优溶剂组合,并在此条件下研究提取温度、液料比和提取次数对红毛藻类胡萝卜素提取的影响。混料实验设计出最优溶剂组合为V(乙醇)∶V(丙酮)=1∶1,确定的最佳提取工艺为提取温度40℃、液料比20∶1(mL/g)和提取3次。在最佳提取条件下和混料模型预测的类胡萝卜素提取量分别为2.264、2.202 mg/g,研究结果与预测值的偏差率小于5%。该研究基于混料设计建立的方法及模型真实有效,为红毛藻中类胡萝卜素的研究提供了理论依据和技术支持。

Bangia fusco-purpurea is one of the natural sources of carotenoids.In this study,Bangia fusco-purpurea was used as a raw material to determine the optimal condition for the extraction of carotenoids.The regression model between the ratio of extraction reagent and carotenoids content was established by mixture design to obtain the optimal solvent combination for carotenoids extraction from Bangia fusco-purpurea,and the effects of extraction temperature,liquid-solid ratio,and extraction times on carotenoids extraction were studied.The optimal solvent combination of V(ethanol)∶V(acetone)=1∶1 was designed through the mixing experiments,and the optimal extraction process was determined as extraction temperature of 40℃,liquid-solid ratio of 20∶1(mL/g),and 3 times of extraction.The carotenoids contents under the optimal extraction condition and that predicted by the mixture model were 2.264 mg/g and 2.202 mg/g,respectively.The deviation rate between the research results and the predicted value was less than 5%.The method and model established in this study based on mixture design are real and effective,which provides theoretical basis and technical support for the study of carotenoids from Bangia fusco-purpurea.