马铃薯根际耐盐碱细菌T11的筛选及促生效果研究

Screening of Saline-alkali Tolerant Bacteria T11 in Potato Rhizosphere and Their Growth Promotion Effect

为了筛选马铃薯根区的耐盐碱细菌,探索其促生效果,本研究以马铃薯根区土壤细菌为研究对象,通过划线法分离细菌,设置不同NaCl浓度(100 g/L、120 g/L、140 g/L、160 g/L、180 g/L)和pH梯度(9、10、11、12、13、14),筛选马铃薯根际耐盐碱细菌。利用27F/1492-R引物鉴定细菌的种类,通过测定细菌的生长曲线、去盐去碱和解钾效果、产IAA和产蛋白酶能力,综合分析其促生效果。结果表明:随着pH值的升高,细菌的耐性不同,当pH为13,NaCl浓度为160 g/L时,T11细菌存活,其他细菌不生长;促生效果结果表明,T11菌株具有产IAA、解钾和产蛋白酶能力,去盐碱效果较弱。本研究筛选得到的T11菌株为芽孢杆菌,具有促生效果,为马铃薯专用菌肥开发奠定微生物基础。

In order to screen the saline-tolerant bacteria in the potato root zone and explore their growth-promoting effects,bacteria were used as subjects and isolated by the plate streak method.Different NaCl concentrations(100 g/L,120 g/L,140 g/L,160 g/L,180 g/L)and pH gradients(9,10,11,12,13,14)were set to screen saline-tolerant bacteria in the potato rhizosphere.The 27F/1492-R primers were used to identify the species of bacteria,and their growth-promoting effects were analyzed by measuring the growth curve,the effect of removing salt and alkali,and the ability to produce IAA and protease.The results showed that with the increase of pH value,the tolerance of the bacteria varied.When pH was 13 and the NaCl concentration was 160{g/L},the T11 bacteria survived,while the other bacteria did not grow.The results of the growth promotion assays showed that T11 had the ability to produce IAA and protease,but the effect of removing salt and alkali was weak.The T11 strain belongs to the genus%Bacillus%and has a growth-promoting effect,laying a foundation for the development of a specialized bacterial fertilizer for potatoes.