基于层次分析法、多指标综合评分法结合正交试验优选黑豆汁炮制女贞子的炮制工艺研究

Optimization of processing technology of Fructus Ligustri Lucidi by black bean juice based on analytic hierarchy process,multi-index comprehensive scoring method combined with orthogonal test

目的研究基于层次分析法、多指标综合评分法结合正交试验优选黑豆汁炮制女贞子的炮制工艺。方法建立高效液相色谱法同时测定女贞子中的酪醇、红景天苷、女贞苷、特女贞苷、齐墩果酸的含量,以各指标成分含量为综合评价指标,以黑豆汁用量、闷润时间、炮制时间为考察因素,采用层次分析法确定权重系数,多指标综合评分法结合正交试验法优选黑豆汁炮制女贞子的炮制工艺。结果根据层次分析法计算结果,分别给予红景天苷0.370、特女贞苷0.533、女贞苷0.197的加权系数,方差分析结果表明黑豆汁用量和炮制时间对成分具有显著性影响(P<0.05),闷润时间对结果的影响不显著(P>0.05);综合分析确定黑豆汁炮制女贞子的最佳工艺组合为女贞子每100g加黑豆汁20%,闷润60min,炮制8h。结论基于层次分析法、多指标综合评分法结合正交试验优选黑豆汁炮制女贞子的炮制工艺方法稳定、可靠,有助于测定女贞子中的各成分含量,有助于维持女贞子的质控水平。

Objective To optimize the processing technology of Fructus Ligustri Lucidi(Nvzhenzi in Chinese)by black bean juice based on analytic hierarchy process,multi-index comprehensive scoring method,and orthogonal test.Methods A high performance liquid chromatography(HPLC)method was established for the simultaneous determina-tion of tyrosol,salidroside,specnuezhenide,ligustroflavone,and oleanolic acid in Fructus Ligustri Lucidi.With the con-tent of the indicators as the comprehensive evaluation index and the amount of black bean juice,dampening time,and processing time as the factors,the weighted coefficient was determined by analytic hierarchy process,and the processing technology of Fructus Ligustri Lucidi was optimized by multi-index comprehensive scoring method combined with or-thogonal test.Results According to the results of analytic hierarchy process(AHP),the weighted coefficients of sali-droside,specnuezhenide,and ligustroflavone were 0.370,0.533,0.197,respectively.Variance analysis showed that the amount and processing time of black bean juice had significant effects on the composition(P<0.05),while the influence of moistening time on the results was not significant(P>0.05).Comprehensive analysis showed that the best processing combination for Fructus Ligustri Lucidi with black bean juice was adding 20%black bean juice per 100 grams,moisten-ing for 60 min and processing for 8 h.Conclusion Optimization of processing technology of Fructus Ligustri Lucidi by black bean juice based on analytic hierarchy process,multi-index comprehensive scoring method combined with orthogo-nal test is stable and reliable,which is helpful to determine the content of various components in Fructus Ligustri Lucidi and to maintain the quality control level.