基于质量常数结合颜色精准识别的酒女贞子质量快速评价研究

Rapid evaluation study of wine-processed Ligustri Lucidi Fructus based on quality constant combined with precise color detection

目的以酒女贞子为例,基于质量常数结合颜色精准识别建立一种中药质量快速评价方法。方法以市场收集到的20批酒女贞子饮片为研究对象,测定饮片质量(m)和红景天苷含量(c);通过计算得到质量常数(A)与百分质量常数(A%),并据此建立一种质量常数评价方法;采用色度仪对样品粉末色度(L*、a*、b*)进行快速检测,将其与A、c进行相关性分析,在中药颜色精准识别后关联质量内涵,建立基于色度的快速质量评价法。结果根据质量常数评价法划分为三组:优组(A≥0.047,2批),良组(0.028≤A<0.047,5批),合格组(A<0.028,12批)。样品色度值L*、a*、b*与A、c呈负相关(P<0.05);通过色度值实现了酒女贞子质量的快速评价,将样品分为两组,即上品(含优组、良组)L*≤27.68,b*≤11.49,a*≤5.84,普品(含合格组)L*>27.68,b*>11.49,a*>5.84。结论以酒女贞子为例,建立了一种包含性状和成分双重特征的质量常数评价法;同时为色度值赋予质量内涵,建立了一种“更快、更准、更便捷”的色度识别评价法。

Objective To establish a rapid evaluation method for Chinese herbal medicines quality based on quality constants combined with precise color detection,using the example of wine-processed Ligustri Lucidi Fructus.Methods Taking 20 batches of wine-processed Ligustri Lucidi Fructus collected from the market as the research objects,the quality(m)and salidroside content(c)of the wine-processed Ligustri Lucidi Fructus were determined;the quality constant(A),and percentage quality constant(A%)were calculated and a quality constant evaluation method was established accordingly;the chromaticity(L*,a*,b*)of the wine-processed Ligustri Lucidi Fructus powder was rapidly detected by chromaticity meter,and its correlation with A and c was analyzed.After the accurate identification of traditional Chinese medicine color,the rapid quality evaluation method based on chromaticity was established.Results According to the quality constant evaluation method,they were divided into three groups:excellent group(A≥0.047,two batches),good group(0.028≤A<0.047,five batches),and qualified group(A<0.028,12 batches).The colorimetric indicators(L*,a*,b*)of the samples were negatively correlated with A and c(P<0.05);the rapid quality evaluation of wine-processed Ligustri Lucidi Fructus was established through chromaticity values,and the quality of wine-processed Ligustri Lucidi Fructus samples were quickly evaluated,being divided into two groups:superior grade(including excellent grade and good grade)with L*≤27.68,b*≤11.49,a*≤5.84,and general grade(including qualified grade)with L*>27.68,b*>11.49,a*>5.84.Conclusion Quality constant evaluation method including both traits and components has been established with wine-processed Ligustri Lucidi Fructus as an example.At the same time,quality connotations are assigned to chromaticity values,and a“faster,more accurate,and more convenient”chromaticity recognition evaluation method is established.