低离子凝聚胺法与抗人球蛋白微柱凝胶法开展交叉配血试验在输血检验中的价值对比分析

Comparative Analysis of the Value of Cross Matching Blood Test Between Low Ion Coagulated Amine Method and Anti-human Globulin Microcolumn Gel Method in Blood Transfusion

目的研究在临床输血检验工作当中,应用低离子凝聚胺法、抗人球蛋白微柱凝胶法开展交叉配血试验的价值。方法非随机选取2021年8月—2022年12月如东县人民医院接受交叉配血试验后输血治疗的199例患者为研究对象。应用低离子凝聚胺法、抗人球蛋白微柱凝胶法进行交叉配血试验,对比两种方法的配血时间、检测结果、不规则抗体阳性检测情况。结果低离子凝聚胺法的配血时间短于抗人球蛋白微柱凝胶法,差异有统计学意义(P<0.05);抗人球蛋白微柱凝胶法主侧阳性率为4.52%(9/199)、次侧阳性率为6.03%(12/199)、总阳性率为10.55%(21/199),均高于低离子凝聚胺法的0.50%(1/199)、2.01%(4/199)、2.51%(5/199),差异有统计学意义(χ^(2)=6.565、4.168、10.534,P均<0.05);两种检测方法的不规则抗体阳性率对比,差异无统计学意义(P均>0.05)。结论低离子凝聚胺法的配血时间较短,抗人球蛋白微柱凝胶法的阳性检出率高,两种方式各具优势,临床可根据实际情况选用。

Objective To study the value of cross matching blood test with low ion coagulated amine method and anti-human globulin microcolumn gel method in clinical blood transfusion test.Methods A total of 199 patients who received blood transfusion treatment after cross-matching blood test in Rudong County People's Hospital from August 2021 to December 2022 were non-randomly selected as the study subjects.Cross matching blood test was carried out by low ion agglutination amine method and anti-human globulin microcolumn gel method.The blood matching time,detection results and irregular antibody positive detection of the two methods were compared.Results The blood matching time of low ion condensed amine method was shorter than that of anti-human globulin microcolumn gel method,and the difference was statistically significant(P<0.05).The positive rate of anti-human globulin microcolumn gel method was 4.52%(9/199)in the main side,6.03%(12/199)in the secondary side,and 10.55%(21/199)in the total,which were higher than 0.50%(1/199),2.01%(4/199),2.51%(5/199)of low ion agglomerated amine method,and the differences were statistically significant(χ^(2)=6.565,4.168,10.534,all P<0.05).There were no statistically significant differences in the positive rate of irregular antibody between the two detection methods(all P>0.05).Conclusion The blood matching time of low ion condensed amine method is short,and the positive detection rate of anti-human globulin microcolumn gel method is high.The two methods have their own advantages,and can be selected according to the actual situation in clinic.