冷休克蛋白RBM3在幼鼠肾脏缺血再灌注损伤中的作用研究

Role of cold shock protein RBM3 in renal ischemia reperfusion injury in young rats

目的建立幼鼠肾脏缺血再灌注损伤(RIRI)模型,探讨亚低温环境诱导的冷休克蛋白RBM3对RIRI的作用。方法将7 d SD幼鼠24只随机分为正常对照组(NC组)、假手术组(Sham组)、RIRI未亚低温组(NH组)、RIRI亚低温组(TH组),每组6只。建立肾脏RIRI模型,进行全身亚低温治疗;检测血肌酐(SCr)、半胱氨酸蛋白酶抑制剂C(CysC)水平;取左肾组织行HE染色,观察病理学变化,取右肾组织用qPCR检测YAP1和NRF2基因表达情况;用Western Blot检测RBM3蛋白表达量。结果NC组与Sham组的SCr、CysC水平比较,差异无统计学意义(P>0.05);NH组与TH组的SCr、CysC水平显著高于NC组,NH组的SCr、CysC水平显著高于TH组(P<0.05)。NH组和TH组的病理组织评分高于NC组,NH组的病理组织评分高于TH组(P<0.05)。TH组的YAP1、NRF2基因表达显著高于NC组和NH组(P<0.05)。TH组的RBM3蛋白表达量明显高于NC组和NH组(P<0.05)。结论全身亚低温诱导的RBM3对幼鼠RIRI有一定的保护作用,这种保护作用可能与低温诱导的RBM3降低了炎症反应有关。

Objective To establish a model of renal ischemia reperfusion injury(RIRI)in young rats,and to explore the effect of cold shock protein RBM3 induced by mild hypothermia on RIRI.Methods Twenty-four 7 d SD young rats were randomly divided into normal control group(NC group),sham operation group(Sham group),RIRI non-hypothermia group(NH group)and RIRI hypothermia group(TH group),with 6 rats in each group.Renal RIRI model was established and whole-body mild hypothermia therpy was performed;the levels of serum creatinine(SCr)and Cystatin C(CysC)were detected;the left renal tissue was taken for HE staining to observe the pathological changes,and the right renal tissue was taken to detect the expression of YAP1 and NRF2 genes by qPCR;the expression of RBM3 protein was detected by Western Blot.Results There were no significant differences in the levels of SCr and CysC between the NC group and the Sham group(P>0.05);the levels of SCr and CysC in the NH group and the TH group were significantly higher than those in the NC group,and the levels of SCr and CysC in the NH group were significantly higher than those in the TH group(P<0.05).The pathological tissue score of the NH group and the TH group was higher than that of the NC group,and the pathological tissue score of the NH group was higher than that of the TH group(P<0.05).The expression of YAP1 and NRF2 genes in the TH group were significantly higher than those in the NC group and the NH group(P<0.05).The expression of RBM3 protein in the TH group was significantly higher than that in the NC group and the NH group(P<0.05).Conclusion RBM3 induced by systemic mild hypothermia has a protective effect on RIRI in young rats.This protective effect may be related to the reduction of inflammatory response by RBM3 induced by hypothermia.