海南省文昌鸡主要养殖地区鸡毒支原体和鸡滑液囊支原体的血清学调查与分析

Serological Investigation and Analysis of Mycoplasma gallisepticum and Mycoplasma synovialum in Main Breeding Areas of Wenchang Chickens in Hainan Province

为探明海南省文昌鸡主要养殖地区鸡毒支原体(MG)和鸡滑液囊支原体(MS)的感染情况,采集来自海口、文昌、琼海、儋州4个文昌鸡主要养殖地区的13个不同规模鸡场未经MG、MS疫苗免疫的文昌鸡血样1726份,采用酶联免疫吸附试验进行MG、MS血清抗体检测。结果显示,MG和MS的抗体总阳性率分别为42.00%和31.75%;4个地区以琼海市的MG抗体阳性率最高,达到68.00%;儋州市的MS抗体阳性率最高,达到36.67%;冬季MG的感染率较高,抗体阳性率达47.48%,夏、秋两季MS的感染率较高,抗体阳性率分别为37.85%和35.87%;成年文昌鸡MG和MS的抗体阳性率高于雏鸡和育成鸡(P<0.05),分别为74.45%和59%;小规模场的MG、MS抗体阳性率最高,分别为54.10%和46.45%;不同养殖类型的文昌鸡以商品代蛋鸡MG的抗体阳性率最高,达到83.33%,商品代肉鸡MS的抗体阳性率最高,达到37.04%。结果表明,海南省文昌鸡主要养殖地区普遍存在MG、MS感染。提示各养殖地区应针对性地加强对文昌鸡MG和MS的防控,以减少疫病发生。

In order to investigate the infections of Mycoplasma gallisepticum(MG)and Mycoplasma synovialum(MS)in Wenchang chicken breeding area of Hainan province,1726 blood samples of Wenchang chickens without MG and MS vaccine were collected from 13 chicken farms of different scales in four major breeding areas of Wenchang chickens in Haikou,Wenchang,Qionghai and Danzhou.Serum antibodies to MG and MS were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that the total positive rates of MG and MS were 42.00%and 31.75%,respectively.The positive rate of MG antibody in Qionghai was the highest,reaching 68.00%.The positive rate of MS antibody was the highest in Danzhou(36.67%).The incidence of MG was high in winter,and the positive rate of antibody was 47.48%.The incidence of MS was high in summer and autumn,and the positive rates of antibody were 37.85%and 35.87%.The antibodies positive rates of MG and MS in adult Wenchang chickens were higher than those in chicks and growing chickens(P<0.05),which were 74.45%and 59%,respectively.The positive rates of MG and MS antibody in small scale farms were 54.10%and 46.45%,respectively.The positive rate of MG antibody in commercial layer was the highest in Wenchang chickens,reaching 83.33%,and the positive rate of MS antibody in commercial broilers was the highest,reaching 37.4%.The results showed that MG and MS infections were common in the main chicken breeding areas of Wenchang,Hainan province.It is suggested that all breeding areas should strengthen the prevention and control of MG and MS in Wenchang chickens to reduce the incidence of disease.