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果糖联合氧嗪酸钾构建大鼠高尿酸血症模型的研究

Study on the Establishment of Hyperuricemia Model in Rats by Fructose Combined with Potassium Oxonate
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摘要 目的利用果糖联合氧嗪酸钾构建大鼠高尿酸血症(HUA)模型,为HUA模型的建立和药物研发提供参考。方法5%果糖溶液联合100 mg·(kg·d)^(-1)氧嗪酸钾溶液构建大鼠HUA模型,造模4周后观察尿酸(UA)、肝功能[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)]、肾功能(BUN、Scr)、血脂[血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、空腹血糖(FPG),利用Lasso回归筛选变量并进行多元线性回归分析。4周后,实验组大鼠停止摄入5%果糖水,并随机分为氧嗪酸钾组和无氧嗪酸钾组,在第8周观察大鼠尿酸、肝功能、肾功能和血脂的恢复状况,以及氧嗪酸钾对各指标的作用。结果第4周末,实验组大鼠饲料消耗量低于对照组,果糖水摄取量、ALT、AST、BUN和Scr均高于对照组(P均<0.05)。Lasso回归筛选的变量为ALT、BUN、Scr,多元线性回归分析方程为YUA=-383.717+0.676×ALT+31.551×BUN+10.589×Scr。停止饮用果糖水后,第6周氧嗪酸钾组大鼠的饲料消耗量高于对照组,BUN高于对照组大鼠和无氧嗪酸钾组(P均<0.05)。第8周无氧嗪酸钾组大鼠的饮水量高于对照组(P<0.05)。肝功能、肾功能、血脂和其他指标差异均无统计学意义(P均>0.05)。结论5%果糖溶液联合100 mg·(kg·d)^(-1)氧嗪酸钾溶液成功构建了肝脏、肾脏损伤较轻的大鼠HUA模型。 Objective The hyperuricemia(HUA)model of rats was established by fructose combined with potassium oxonate to provide reference for the establishment and drug development of HUA model.Methods The HUA model in rats was established with 5%fructose combined with 100 mg·(kg·d)^(-1)potassium oxonate solution.After 4 weeks of modeling,the indicators of uric acid(UA),liver function(ALT,AST),kidney function(BUN,Scr),blood lipids(TC,TG,HDL-C,LDL-C)and fasting blood glucose(FPG)were observed.Lasso regression was used to screen variables and multiple linear regression was used to analyze variables.After 4 weeks,the experimental rats stopped consuming 5%fructose water and was randomly divided into a potassium oxonate group and a non-potassium oxonate group observe the recovery of uric acid,liver and kidney function and blood lipids at week 8,as well as the effects of potassium oxonate on various indicators.Results The results of experiment showed that at the end of the 4th week,the feed consumption of experimental rats was lower than that of control rats,while the fructose water intake,ALT,AST,and Scr were higher than those of control rats(P all<0.05).The variables selected by Lasso regression were ALT,BUN,and Scr,while the equation for multiple linear regression analysis was YUA=-383.717+0.676×ALT+31.551×BUN+10.589×Scr.After stopping drinking fructose water,the feed consumption of potassium oxonate group in rats was significantly higher than that of control rats at the 6th week,and the BUN was higher than that of control rats and non-potassium oxonate group in rats(P all<0.05).At week 8,the water intake of non-potassium oxonate group in rats was higher than that of control rats(P<0.05).There were no statistical significance in liver and kidney function,blood lipids and other indicators(P all>0.05).Conclusion The HUA model in rats was successfully established by 5%fructose combined with 100 mg·(kg·d)^(-1)potassium oxonate with liver and kidney functions damaged in some extent.
作者 王燕 彭玥 杨宇轩 郭忠琴 WANG Yan;PENG Yue;YANG Yuxuan;GUO Zhongqin(Ningxia Medical University,School of Public Health,Yinchuan 750004,China;Key Laboratory of Environmental Factors and Chronic Disease Control,Yinchuan 750004,China)
出处 《宁夏医科大学学报》 2024年第11期1102-1108,共7页 Journal of Ningxia Medical University
基金 宁夏自然科学基金项目(2021AAC03129)。
关键词 高尿酸血症 果糖 氧嗪酸钾 血脂 hyperuricemia fructose potassium oxonate blood lipid
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