富锶水对老龄小鼠运动能力的改善作用及机制研究

Effect and Mechanism of Strontium-Rich Water on the Exercise Ability of Aged Mice

目的探讨富锶水干预对老龄小鼠机体运动能力的影响及其作用机制。方法采用20月龄SPF级雄性C57BL/6老龄小鼠16只,随机分为富锶水组和对照组,每组8只,对照组采用纯净水,富锶水组采用富锶水(6.11 mg/L)替代饮用水,2组均自由饮用,每隔2 d更换1次水瓶,连续干预8周;测量2组体质量及心、肝、肾等脏器的重量,HE染色法检测2组心脏、肝脏、肾脏、肌肉病理改变,拉力测试仪检测2组肌肉拉力值;旷场实验检测2组自主活动能力,免疫组织化学法检测2组肌肉中TNF-α、IL-1β、IL-6炎症指标表达情况;Western blot法检测2组NF-κB信号通路的蛋白相对表达量。结果与对照组比较,富锶水组体质量变化差异无统计学意义(P>0.05);与对照组比较,富锶水组心脏、肝脏、肾脏等脏器差异无统计学意义(P>0.05);与对照组比较,富锶水组心、肝、肾的病理形态均未产生明显变化;与对照组比较,老富锶水组肌肉组织中肌细胞排列整齐度、紧密程度优于对照组;与对照组比较,富锶水组肌肉拉力(十次拉力)的均值明显增大(P<0.05);与对照组比较,富锶水组5 min持续位移距离及平均速度明显增加(P<0.05),静止时间缩短(P<0.05);与对照组比较,富锶水组肌肉组织中TNF-α、IL-1β、IL-6表达水平显著降低(P<0.05);与对照组比较,富锶水组肌肉组织中NF-κB及p-NF-κB蛋白相对表达量显著降低(P<0.05)。结论富锶水通过抑制老龄小鼠肌肉中NF-κB炎症信号通路的激活,降低肌肉炎症反应,抑制炎症因子表达,提高老龄小鼠肌肉拉力及自主活动能力,可能是富锶水改善老龄小鼠机体运动能力的作用机制之一。

Objective To investigate the effects and underlying mechanisms of strontium-rich water on exercise ability of aged mice.Methods A total of 16 male C57BL/6 aged mice at 20 months of age were divided into strontium-rich water group and control group,with 8 mice in each group.The strontium-rich water group was provided with strontium-rich water(6.11 mg/L)instead of drinking water,and the control group was given pure water.Both groups were free to drink,with water bottles being changed every two days for 8 consecutive weeks.Body weight and the weight of organs such as the heart,liver and kidney were detected.The pathological changes of heart,liver,kidney and muscles were detected by HE staining.Tension tester was used to measure the muscle tension of aged mice.The autonomous activity of aged mice was detected by open field experiment.The expression of inflammatory markers TNF-α,IL-6 and IL-1βin the muscles were detected by immunohistochemistry.The levels of NF-κB were detected by Western blot in both groups.Results Compared with the control group,there was no significant effect on body weight in the strontium-rich water group(P>0.05);there was no significant effect on the weight of organs such as heart,liver,and kidney of aged mice in the strontium-rich water group(P>0.05).The intervention of strontium-rich water showed no significant changes in the pathological morphology of the heart,liver,and kidneys of aged mice,but compared with the control group,the strontium-rich water group showed better muscle cell arrangement and tightness.Compared with the control group,the average muscle tension in the strontium-rich water group significantly increased(P<0.05).The intervention of strontium rich water can significantly increase the displacement distance and average velocity of aged mice over 5 minutes,and reduce their resting time(P<0.05).After intervention with strontium-rich water,the expressions of inflammatory factors TNF-α,IL-1βand IL-6 in muscles significantly reduced(P<0.05);the expressions of key proteins NF-κB and p-NF-κB in the inflammatory signaling pathway significantly reduced(P<0.05).Conclusion Strontium-rich water may improve the physical exercise capacity of aged mice by inhibiting the activation of the NF-κB inflammatory signaling pathway in muscle tissue,reducing muscle inflammation,suppressing the expression of inflammatory factors,and enhancing muscle tension and spontaneous activity in aged mice.This may be one of the mechanisms by which strontium-rich water improve the exercise ability of aged mice.