昆布多糖的提取工艺优化及其对小鼠抗疲劳能力的研究

Optimization of laminarin extraction and its effects of anti-fatigue on mice

采用响应面法对昆布多糖的提取工艺进行优化,并通过小鼠负重游泳试验评价昆布多糖对小鼠抗疲劳能力的影响。结果表明:在液料比21∶1、复合酶添加量1.3%、酶解温度65℃、酶解时间181 min的条件下,昆布多糖的提取率可达到11.48%。各生化指标结果显示:与对照组相比,昆布多糖各剂量组小鼠负重游泳时间显著延长(P<0.05),血乳酸、血尿素氮含量显著降低(P<0.05),中、高剂量组肝/肌糖原含量显著增加(P<0.05),结果说明昆布多糖对小鼠具有较好的抗疲劳作用,是一种较有前景的运动功能性物质。

The response surface method was used to optimize the extraction process of laminarin(Lam),and the effect on the anti-fatigue ability of mice was evaluated through a weight-bearing swimming test.The results showed that under the conditions of a liquid-solid ratio of 21∶1,multi-enzyme dosage of 1.3%,enzymolysis temperature of 65℃,and enzymolysis time of 181 minutes,the extraction rate of Lam could reach 11.48%.Various biochemical indicators indicated that compared to the control group,the weight-bearing swimming time of mice in each dose group of Lam were significantly increased(P<0.05),while the level of blood lactate and blood urea nitrogen were significantly decreased(P<0.05).Additionally,the hepatic/muscle glycogen content in the medium and high dose groups was also significantly increased(P<0.05).These findings indicate that Lam had a good anti-fatigue effect on mice and are a promising functional product for exercise.