低共熔溶剂法提取葡萄皮籽多酚、黄酮及其抗氧化性研究

A Study on the Extraction of Polyphenols and Flavonoids from Grape Skin Seeds and Its Antioxidant Properties by Deep Eutectic Solvent Process

以和田红葡萄皮籽为原材料,采用低共熔溶剂法提取其多酚和黄酮类物质,并与80%乙醇为溶剂提取效果进行比较试验。以黄酮和多酚提取量为指标,经单因素试验和响应面优化试验,获得了最优的提取工艺条件,并分析了提取液对DPPH、ABTS+自由基的清除能力。结果表明,低共熔溶剂的组成为氯化胆碱-乳酸(1∶2),含水量为20%,最佳工艺条件为提取温度50℃、提取时间35 min、料液比1∶26(g/mL),此时黄酮平均提取量为57.46 mg/g,多酚平均提取量为79.81 mg/g。葡萄皮籽提取物表现出显著的抗氧化活性。

This research utilized Hontan red grape peels and seeds as raw materials,and employed a deep eutectic solvent(DES)method to extract polyphenols and flavonoids,comparing the results with extraction using 80%ethanol as the solvent.Through single-factor experiments and response surface methodology optimization,the optimal extraction conditions were determined,and the scavenging ability of the extract on DPPH and ABTS+free radicals was analyzed.The results indicated that the optimal DES composition was choline chloride-lactic acid(1∶2)with a water content of 20%.The best extraction conditions were an extraction temperature of 50℃,an extraction time of 35 minutes,and a solid-to-liquid ratio of 1∶26(g/mL).Under these conditions,the average extraction yield of flavonoids was 57.46 mg/g,and the average extraction yield of polyphenols was 79.81 mg/g.The extract from grape peels and seeds exhibited significant antioxidant activity.