摘要
该文探究传统发酵乳源短乳杆菌(Lactobacillus brevis)HQ1-1的基因组基本特征和糖代谢表型。经PacBio平台对短乳杆菌HQ1-1进行全基因组测序,并对基因组进行功能分类和注释。结果显示,短乳杆菌HQ1-1包含1条长度为2376708bp的环状染色体和7个大小不同的质粒DNA,编码2268个基因。通过KEGG数据库注释到参与代谢过程的基因有860个,其中,碳水化合物代谢相关基因的数量为142个,占比为16.51%。进一步用CAZy数据库注释到了糖苷水解酶类基因114个,占碳水化合物相关基因总数的45.24%。通过OmniLog微阵列表型芯片分析发现,短乳杆菌HQ1-1对L-阿拉伯糖、D-木糖、D-核糖、L-来苏糖等碳源的利用效率相对更高。因此,发酵乳源短乳杆菌HQ1-1基因组中包含碳水化合物代谢相关基因的数量较多,能够利用多种碳源。该文全面阐述了短乳杆菌HQ1-1基因组特征和代谢表型,为今后开发功能性发酵食品提供新型乳酸菌发酵剂。
In this paper,the basic characteristics of the genome and glycometabolic phenotype of Lactobacillus brevis HQ1-1 from traditional fermented milk were explored.The whole genome sequencing of Lactobacillus brevis HQ1-1 was conducted by PacBio platform,and the functional classification and annotation of the genome were performed.The results showed that Lactobacillus brevis HQ1-1 carried a circular chromosome with a length of 2376708 bp and 7 plasmids DNA of different sizes,encoding 2268 genes.There were 860 genes annotated by KEGG database involved in metabolic process,among them 142 genes were related to carbohydrate metabolism,accounting for 16.51%.Further,114 glycoside hydrolases genes were annotated using CAZy database,accounting for 45.24%of the total number of carbohydrate-related genes.According to OmniLog microarray phenotype chip analysis,Lactobacillus brevis HQ1-1 was found to have relatively higher efficiency of utilizing carbon sources like L-arabinose,D-xylose,D-ribose,L-lyxose.Therefore,the genome of Lactobacillus brevis HQ1-1 contains a large number of genes related to carbohydrate metabolism,possessing utilization capacity for various carbon source.The paper comprehensively elaborates the genome characteristics and metabolic phenotypes of Lactobacillus brevis HQ1-1,and provides novel lactic acid bacteria starter for the development of functional fermented food in the future.
作者
吴青海
三月
萨初拉
郭煜
王力伟
王莉梅
杨静
其布日
WU Qinghai;SAN Yue;SA Chula;GUO Yu;WANG Liwei;WANG Limei;YANG Jing;QI Buri(Biotechnology Institute,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Department of Catering and Food,Inner Mongolia Business and Trade Vocational College,Hohhot 010070,China)
出处
《食品与发酵科技》
CAS
2024年第6期1-7,21,共8页
Food and Fermentation Science & Technology
基金
内蒙古农牧业青年创新基金项目(2023QNJJM03)。
关键词
传统发酵乳
短乳杆菌HQ1-1
全基因组测序
功能基因
糖代谢表型
traditional fermented milk
Lactobacillus brevis HQ1-1
whole genome sequencing
functional gene
glycometabolic phenotype