库尔勒香梨树皮内生菌结构多样性与影响因子分析

Structural diversity and influencing factors of endophytes in the bark of Korla pear

腐烂病是一种主要发生于库尔勒香梨树皮内的严重病害,该研究通过高通量测序方法,对库尔勒香梨树皮内生菌的组成结构和多样性及影响因子进行研究,旨在探明库尔勒香梨树皮内生菌动态变化及受腐烂病和营养元素的影响。结果表明,库尔勒香梨内生细菌分布于67个门555个属,真菌分布于9个门405个属。在属水平,细菌优势菌为大肠埃希菌(Escherichia)、醋酸杆菌属(Acetobacter)、链霉菌属(Streptomyces)、盐水球菌属(Salinicoccus),真菌优势菌为条锈菌属(Puccinia)、球囊霉属(Rhizophagus)、根霉属(Rhizopus)。库尔勒香梨树皮内生菌受腐烂病和营养元素显著影响。营养元素氮、磷、钾与部分内生菌存在不同程度的相关性,树皮内生真菌与氮、钾元素相关性更大。库尔勒香梨树皮内生菌的功能主要被注释到新陈代谢(metabolism)、有机系统(organic system)、环境信息加工(environmental information processing)3大通路,发病树皮的3大通路丰度高于健康树皮。该研究明确了新疆库尔勒香梨树皮内生菌群落结构与多样性,患腐烂病与健康树皮有一定的差异,内生菌与营养元素间存在相互作用,树皮中存在一定的有益微生物和可供开发利用的有益功能菌群,用于库尔勒香梨腐烂病的生物防治、促进果业发展。

In this study,the composition structure and influencing factors of endophytic bacteria in the bark of Korla pear were studied by high-throughput sequencing method,aiming to find out the diversity and community structure of endophyte,as well as the effects of rot disease and nutrient elements on endophyte.The results showed that endophytes were distributed in 67 phyla and 555 genera,while fungi were distributed in 9 phyla and 405 genera.At the genus level,the dominant bacteria were Escherichia,Acetobacter,Streptomyces and Salinicoccus.The dominant endophytic fungi were Puccinia,Rhizophagus and Rhizopus.The endophyte in Korla pear bark were significantly affected by Valsa canker and nutrients.Nitrogen,phosphorus and potassium had different correlations with some endophytes.The functions of endophytes in the bark of Korla pear were mainly annotated into three major pathways:metabolism,organic system,and environmental information processing.The abundance of three pathways in diseased bark was higher than that in healthy bark.In this study,the community structure and diversity of endophyte in the bark of Korla pear in Xinjiang were identified,and there were certain differences between the bark with Valsa canker and the healthy bark.There were some interactions between endophyte and nutrient elements,and there were some beneficial microorganisms in the bark.