疏肝散对慢性肝炎大鼠模型的疗效研究

Study on the Efficacy of Shugansan on a Rat Model of Chronic Hepatitis

研究中药复方制剂疏肝散对慢性肝炎大鼠的疗效作用。采用皮下注射40%四氯化碳(CCl_(4))溶液复制慢性肝炎大鼠模型。本试验选用60只雄性SD大鼠,随机分为对照组、模型组、疏肝散低、中、高剂量组和阳性对照组,每组10只。除对照组外,其余各组按1.0 mL/kg皮下注射40%CCl_(4)溶液,对照组皮下注射等体积的大豆油,2次/周,连续10周;同时,从实验第一天起灌胃给药,各给药组按10 mL/kg灌胃给予相应剂量药液,对照组和模型组灌胃等体积纯水,每天给药1次,连续10周。试验期间观察大鼠食欲、状态、精神情况,末次给药后禁食12 h后采血、解剖。检测各组大鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(ALT)、Ⅲ型胶原蛋白(Col-Ⅲ)和Ⅳ型胶原蛋白(Col-Ⅳ)、白介素-1β(IL-1β)和白介素-6(IL-6)的表达情况,计算肝脏脏器系数,观察肝脏组织病理情况。结果显示,与对照组比较,模型组血清中ALT、ALT、Col-Ⅲ、Col-Ⅳ、IL-1β、IL-6含量、肝脏脏器系数均明显或显著升高(P<0.05或者P<0.01),肝脏组织空泡变形明显;与模型组比较,疏肝散各剂量组和阳性对照组均能明显或显著降低大鼠血清ALT、AST、Col-Ⅲ、Col-Ⅳ、IL-1β、IL-6含量、肝脏脏器系数和缓解肝脏组织病变程度。综上所述,中药复方制剂疏肝散对慢性肝炎大鼠模型有明显的疗效作用。

Investigate the therapeutic effect of Shugan Powder,a traditional Chinese medicine compound,on chronic hepatitis in rats.A chronic hepatitis rat model was established using subcutaneous injection of 40%carbon tetrachloride(CCl_(4))solution.Sixty male Sprague-Dawley rats were randomly divided into control,model,low,medium,high dose Shugan Powder groups,and positive control groups,with 10 rats in each group.Except for the control group,the other groups were injected subcutaneously with 1.0 mL/kg of 40%CCl_(4) solution twice a week for 10 weeks;simultaneously,from the first day of the experiment,they were given medication by gavage.Each treatment group was given the corresponding dose of the drug solution at 10 mL/kg by gavage,while the control and model groups were given an equal volume of pure water by gavage,once a day for 10 weeks.During the trial,the rats'appetite,condition,and mental state were observed.After the last administration,the rats were fasted for 12 hours before blood was drawn and dissection was performed.The expression of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),type Ⅲ collagen(Col-Ⅲ)and type Ⅳ collagen(Col-Ⅳ),interleukin-1β(IL-1β),and interleukin-6(IL-6)in the serum of rats from each group was detected,the liver organ coefficient was calculated,and the liver tissue pathology was observed.The results showed that compared with the control group,the model group had significantly or markedly increased levels of ALT,AST,Col-Ⅲ,Col-Ⅳ,IL-1β,IL-6,and liver organ coefficient in the serum(P<0.05 or P<0.01),and obvious vacuolar degeneration in the liver tissue;compared with the model group,all dose groups of Shugan Powder and the positive control group significantly or markedly reduced the levels of serum ALT,AST,Col-Ⅲ,Col-Ⅳ,IL-1β,IL-6,liver organ coefficient,and alleviated the degree of liver tissue lesions.In summary,the traditional Chinese medicine compound Shugan Powder has a significant therapeutic effect on the chronic hepatitis rat model.