超高效液相色谱法测定北沙参中花椒毒素含量

为建立北沙参中花椒毒素含量的测定方法,采用超高效液相色谱,色谱柱为ACQUITY UPLC^(®)HSS T31.8μm(2.1×100 mm×1.8μm),流动相为50%甲醇乙腈-0.1%磷酸溶液,流速0.3 mL/min,柱温30℃,检测波长323 nm,进样量为1μL。在该色谱条件下,在2.005~80.217μg/mL范围内,北沙参中花椒毒素浓度与峰面积线性关系良好(R=0.9998)。方法精密度RSD为0.23%、重复性RSD为0.87%、稳定性RSD为0.91%、加样回收率在97.0%~105.5%(RSD=2.6%)。该方法快速、准确、便捷,可应用于北沙参中花椒毒素含量的测定。

To establish a method for the determination of Zanthoxytoxin in Glehniae Radix.Ultra-performance liquid chromatography was used.The column was ACQUITY UPLC^(®)HSS T31.8μm(2.1×100 mm×1.8μm),the mobile phase was 50%methanol acetonitrile-0.1%phosphoric acid solution,the flow rate was 0.3 mL/min,the column temperature was 30℃,the detection wavelength was 323 nm,and the injection volume was 1μL.Under the chromatographic conditions,there was a good linear relationship between the concentration of Zanthoxytoxin and peak area in the range of 2.005~80.217μg/mL(R=0.9998).The method precision RSD was 0.23%,the repeatability RSD was 0.87%,the stability RSD was 0.91%,and the sample addition recovery was between 97.0%~105.5%(RSD=2.6%).The method is rapid,accurate and convenient,and thereby can be applied to determine the content of Zanthoxytoxin in Glehniae Radix.