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无背景差分超声分子成像诊断前列腺癌的实验研究

Diagnosis of Prostate Cancer Using Background Free Differential Ultrasound Molecular Imaging:An Experimental Study
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摘要 目的基于前列腺特异性膜抗原(PSMA)靶向超声纳米泡(PSMA-NB),探讨无背景成像差分超声分子成像用于前列腺癌靶向诊断及定位的可行性。材料与方法构建PSMA-NB及非靶向纳米泡(NB)。细胞层面检测PSMA-NBs对人前列腺肿瘤22RV1细胞(PSMA阳性表达)和PC-3细胞(PSMA阴性表达)的寻靶能力。构建人前列腺肿瘤22RV1细胞(n=5)、PC-3细胞(n=5)荷瘤裸鼠模型(n=10),经鼠尾静脉注入PSMA-NB,行原位爆破,采集爆破前后的超声分子图像,利用破坏-补充后处理技术,获取并对比两组差分超声分子成像效果。结果PSMA-NB与NB的粒径大小分别为(363.7±24.4)nm、(236.0±55.2)nm,差异有统计学意义(t=3.19,P=0.007)。细胞寻靶结果显示,PSMA-NB仅黏附在PSMA阳性表达的细胞核周围。动物实验提示,PSMA阳性表达组的差分超声分子图像仅在肿瘤部位显示造影剂高增强区域,且无背景噪声。结论基于PSMA靶向超声纳米泡构建的无背景差分超声分子图像可用于精准靶向诊断及定位PSMA阳性前列腺癌。 Purpose To explore the feasibility of targeted diagnosis and localization of prostate cancer via background free differential ultrasound molecular imaging based on prostate-specific membrane antigen(PSMA)targeted ultrasound nanobubbles(NB).Materials and Methods Targeted PSMA-NB and non-targeted NB were constructed.The targeting ability of PSMA-NB on human prostate tumor 22RV1 cells(PSMA positive expression)and PC-3 cells(PSMA negative expression)was determined in vitro.Ten nude mouse models of human prostate tumor 22RV1 cells(n=5)and PC-3 cells(n=5)were constructed.PSMA-NB was injected into the rat tail vein,and in-situ blasting was performed.Ultrasound molecular images before and after blasting were collected,using destruction supplement post-processing technology to obtain and compare the differential ultrasound molecular imaging effects between the two groups.Results The particle size of PSMA-NB and NB were(363.7±24.4)nm and(236.0±55.2)nm,with statistical difference(t=3.19,P=0.007),respectively.Cell targeting results showed that PSMA-NB only adhered to the nucleus with positive PSMA-expression.Animal experiments indicated that the differential ultrasonic molecular images of PSMA positive expression group only showed the highly enhanced area of contrast agent at the tumor site,with no background noise.Conclusion Background free differential ultrasound molecular images can be used for precise targeted diagnosis and localization of PSMA positive prostate cancer,which is constructed based on PSMA targeted ultrasound nanobubbles.
作者 荣凤 黄钊希 陆柳桂 梁樱子 廖新红 高泳 RONG Feng;HUANG Zhaoxi;LU Liugui;LIANG Yingzi;LIAO Xinhong;GAO Yong(Department of Ultrasound,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处 《中国医学影像学杂志》 CSCD 北大核心 2024年第12期1209-1214,共6页 Chinese Journal of Medical Imaging
基金 国家自然科学基金青年基金项目(82001827)。
关键词 前列腺肿瘤 超声分子成像 差分成像 超声纳米泡 前列腺特异性膜抗原 小鼠 模型 动物 Prostatic neoplasms Ultrasound molecular imaging Differential imaging Ultrasound nanobubbles Prostate-specific membrane antigen Mice,nude Models,animal
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