摘要
线粒体DNA(mitochondrial DNA, mtDNA)拷贝数在不同组织器官中存在一定差异,并与多种重要疾病的发生密切相关。mtDNA拷贝数的稳定性对于维持线粒体功能和细胞代谢至关重要,因此,准确监测mtDNA拷贝数对生物学及生物医学研究具有重要意义。然而,目前常用的分析方法只能对mtDNA拷贝数进行相对定量分析,无法准确评估mtDNA含量。本研究尝试利用高通量测序数据和生物信息学分析软件NOVOPlasty建立了一种定量评估mtDNA拷贝数的方法,并采用该方法对条纹斑竹鲨肌肉与血液组织样品中mtDNA拷贝数开展比较分析。结果表明血液组织的mtDNA含量明显少于肌肉组织的含量,1×全基因组测序深度时肌肉组织的mtDNA拷贝数均值为339,而血液组织约为15,提示肌肉细胞较血细胞的mtDNA含量高约22倍。研究结果表明条纹斑竹鲨肌肉和血液的mtDNA含量存在明显的组织特异性,同时初步验证了该研究建立的mtDNA拷贝数定量分析方法的可行性。该研究可为mtDNA含量相关的生物医学和生理生态研究提供参考资料。
Mitochondrial DNA(mtDNA)copy number varies in different tissues and organs,and is closely related to the occurrence of many important diseases.The stability of mtDNA copy number is essential for maintaining mitochondrial function and cell metabolism.Therefore,accurate monitoring mtDNA copy number is important for biological and biomedical research.However,the current commonly used analytical methods can only perform relative quantitative analysis of mtDNA copy number,and cannot accurately quantify mtDNA content.In this study,a method for quantitative evaluation of mtDNA copy number was established by using high-throughput sequencing data and bioinformatics analysis software NOVOPlasty,and this method was used to quantitatively compare and analyze mtDNA copy number in muscle and blood tissue samples of Chiloscyllium plagiosum.The results showed that the mtDNA content of blood tissue was evidently less than that of muscle tissue.The mean mtDNA copy number of muscle tissue at 1×whole genome sequencing depth was 339,while that of blood tissue was about 15,suggesting that the mtDNA content of muscle cells was about 22 times higher than that of blood cells.The results showed that the mtDNA content in the muscle and blood of C.plagiosum had obvious tissue specificity,and the feasibility of the mtDNA copy number quantitative analysis method established in this study was preliminarily verified.This study can provide reference materials for biomedical and ecophysiological studies related to mtDNA content.
作者
李明僧
张耀仁
徐胜勇
LI Mingseng;ZHANG Yaoren;XU Shengyong(School of Fisheries of Zhejiang Ocean University,Zhoushan 316022,China)
出处
《浙江海洋大学学报(自然科学版)》
CAS
2024年第5期453-458,共6页
Journal of Zhejiang Ocean University:Natural Science
基金
国家自然科学基金项目(32200413)。
关键词
线粒体DNA拷贝数
条纹斑竹鲨
定量分析
组织特异性
高通量测序
mitochondrial DNA copy number
Chiloscyllium plagiosum
quantitative analysis
tissue specificity
high-throughput sequencing