人胚胎囊胚期培养及相关因素研究

Study on culture of human embryo blastocyst and its related factors

目的 分析受精后第3天 (D3)胚胎的细胞数、质量分级与囊胚形成之间的关系 ,比较不同囊胚培养液的囊胚形成率。方法 将体外授精 (IVF)患者胚胎移植后的剩余胚胎54个 ,移入含10 %合成血清代用品(SSS)的囊胚培养液 (IVC -Three、Blastocyst)或人输卵管液 (HTF)中 ,每天观察胚胎卵裂情况 ,直至退化或受精后第7天。 结果 总囊胚形成率29.6% ,囊胚形成率与D3胚胎细胞数呈显著正相关 (r=0.99,P<0.01) ,胚胎质量级别1～2.5级的卵裂球较3～4级者具有更高的囊胚形成率 (34.9%比9.1% )。IVC -Three培养液中囊胚形成率达56.1 % ,与其他两种培养液比较有显著差异 ( χ21=5.77,χ22=3.95,P<0.05)。 结论 序贯培养的囊胚期胚胎培养技术是简便可行的。D3胚胎细胞数及质量分级与囊胚形成有密切关系。IVC -Three培养液是良好的囊胚培养液 。

Objectives To investigate the culture of human embryo blastocyst in vitro and its related factors. Methods The 54 IVF surplus embryos of day 3 from 18 volunteers were transferred to different mediums (IVC-Three medium,blastocyst medium or HTF medium) containing 10%SSS for advanced culture.The growth of embryos was observed until the embryo degeneration or 7 days after fertilization.Results The total blastulation rates of 54 embryos were 29.6%.The positive correlation between the cell number of day 3 embryo and blastulation rate was significant(r=0.99,P<0.01).And the higher blastulation rate was positively related to embryo quality score (34.9% for 1～2.5 degree versus 9.1% for 3～4degree).The blastulation rate of IVC-Three medium was significantly higher than that of blastocyst medium and HTF(χ21=5.77,χ22=3.95,P<0.05).Conclusion Advanced culture of surplus embryo will reach some blastulation.The cell number of day 3 embryo and quality score have some indication to blastulation.IVC-Three blastocyst medium may have potential clinical use for embryo blastocyst culture.