脱氧胶原吡啶交联及尿微量蛋白检测在妊高征早期诊断中的意义

Urinary deoxy-pyridinoline cross-links(DPD), micro-albuminuria (MA),α1-microglobulin (α1-M)in diagnosis of pregnancy-induced hypertension (PIH)

目的 评价脱氧胶原吡啶交联 (DPD)及尿微量白蛋白 (MA)、微球蛋白 (α1 -M)、转铁蛋白 (TRF)在妊高征早期诊断中的意义。 方法 收集101例孕28～32周孕妇清晨空腹中段尿 ,采用化学发光免疫法测定尿DPD ;速率散射比浊法测定MA、α1-M、TRF。按最终是否发生妊高征分成两组。其中妊高征组20例 ,正常妊娠组81例。 结果 妊高征组尿DPD/Cr为11.28±2.42 ;正常组为9.17±2.11,两组差别有显著性意义 (t=3.58,P<0.01) ;尿MA妊高征组为 (0.63±0.30)mg/L,正常组为 (0.42±0.08)mg/L,两组差别有显著性意义 (t=3.15 ,P<0.01) ;尿α1-M妊高征组为 (0.39±0.17)mg/L ,正常组为 (0.87±0.24)mg/L ,两组差别有显著性意义 (t=4.62,P<0.01) ;尿TRF妊高征组为 (0.39±0.17)mg/L,正常组为 (0.33±0.01)mg/L,两组差别无显著性意义 (t=1.72,P>0.05)。 结论 尿DPD、MA、α1-M排泄在妊高征出现之前已经增加 ,测定尿DPD、MA。

Objective To assess the value of urinary deoxy-pyridinoline cross-links(DPD), α1-microglobulin(α1-M), transferrin (TRF) and micro-albuminuria (MA) for prediction of pregnancy- induced hypertension (PIH).Methods Mid-stream morning urine samples were collected from 110 pregnant women at 28-32 weeks for the measurement of DPD, MA, α 1-M and TRF. All subjects were followed up until delivery and were divided subsequently in to two groups: PIH (20 cases) and normal (81 cases) according to the occurrence of PIH. DPD was measured by chemiluminescence-Immunoassay (CLIA), albumin, α1-M, TRF were measured by Rate Nephelometry.Results The urinary DPD/Cr in PIH group was 11.28±2.42,that in the control group was 9.17±2.11 (t=3.58,P<0.01);α 1-M in PIH group was (1.18±0.34 )mg/L and in control group was (0.87±0.24) mg/L (t=4.62,P<0.01);MA in PIH group was (0.63±0.30)mg/L and in control group was (0.33±0.01)mg/L (t=3.15,P<0.01);TRF in PIH group was (0.39±0.17) mg/L and in control group was (0.33±0.01)mg/L (t=1.72, P>0.05).Conclusion The increased urinary excretion of DPD,MA and α 1-M in pregnant women may predict the onset of pregnancy-induced hypertension.