广东海风藤提取物对体外培养海马神经元的影响

The Effect of Kadsura heteroclita on the Cultured Hippocampal Neurons

目的:研究不同剂量广东海风藤提取物(HS_2)对体外培养新生大鼠海马神经元的生长发育的影响。方法:采用体外培养的新生大鼠海马神经细胞,在血清培养液中加入低、中、高剂是HS_2,使之终浓度分别为0.2、1、8mg/ml,通过细胞形态学观察,神经元突起和胞体生长状况的定量比较,不同培养期细胞存活数和培养液中乳酸脱氢酶(LDH)渗漏量的测定,分析研究了HS_2对海马神经细胞生长发育的影响。结果:培养36 h后与对照组比较,中剂量HS_2组海马神经元的突起数目增多,突起长度增加,胞体长径没有显著性变化;低剂量HS_2组与对照组比较,各项指标没有显著差别;高剂量HS_2则明显抑制神经细胞分化。培养4 d、8 d,中剂量HS_2组细胞存活数明显高于对照组,LDH渗漏量明显低于对照组。结论:一定量的HS_2能促进海马神经细胞的生长发育。

Objective:The effects of different concentration Kadsura heteroclita extract(HS2) on the growth of newborn rat hippocampal neurons were observed by means of dissociated cell culture. Methods:The newborn rat hippocampal neurons were cultured with different level HS2. The convert phase microscope was used to analyse the number and the length of neurites,as well as the maximum diameter of hippocampal neurons soma.The survival neurons count and LDH assay were carried out to investigate the effcet of HS2 on the growth and development of neurons in different culture time.Results: After 36h,the number and the length of neurites of 1 mg/ml HS2 group were increased and the maximum diameter of hippocampal neurons soma did not change.The 0.2mg/ml HS2 group did not show statistical difference as compared with the control group, while 8mg/ml HS2 inhibited notably the growth of neurons.The number of survival neurons of 1mg/ml HS2 group was more than that of the control group,and the LDH activity of 1mg/ml HS2 group was lower in different culture time.Conclusion:This study suggested that suitable dose HS2 can promote the growth and development of hippocampal neurons.