姜黄素诱导人卵巢癌细胞株A2780凋亡及其分子机制的研究

Inhibitory Effects of Curcumin on Apoptosis of Human Ovary Cancer Cell Line A2780 and Its Molecular Mechanism

背景与目的:姜黄素(curcumin)是传统中药姜黄的主要有效成分,其选择性抑制肿瘤细胞增殖并诱导其凋亡作用日益成为研究的焦点,但其中所涉及的机制仍然不十分明确。本研究旨在探讨姜黄素对人卵巢癌细胞株A2780的诱导凋亡效应及其分子机制。方法:10～50μmol/L姜黄素分别处理A2780细胞6～24h后,MTT法检测癌细胞的生长活性;流式细胞仪(FCM)和吖啶橙-溴化乙啶荧光染色法检测细胞凋亡;SP免疫组化法检测细胞内NF-κB蛋白(P65)、caspase-3蛋白表达水平。结果:各浓度姜黄素对癌细胞生长抑制率为62.05%～89.24%,DNA直方图上可见亚“G1”峰;荧光显微镜下部分细胞发生凋亡形态学改变,凋亡率为21.5%～33.5%。NF-κB(P65)表达减弱、caspase-3表达增强,并呈时间依赖性。结论:姜黄素能显著抑制卵巢癌细胞的体外生长;上调caspase-3、下调NF-κB蛋白表达、诱导细胞凋亡可能是其作用机制之一。

Background & Objective: Curcumin is the major effective component of curcuma,which is a kind of traditional Chinese medicine. It has been paid more attention to curcumin recently for its specific proliferation inhibition and apoptosis inducing effects on tumor cells;however,the involved mechanisms were not clear. This study was designed to explore the apoptosis inducing effects of curcumin on human ovary A2780 cell line and its related molecular mechanisms. Methods: A2780 cancer cells were treated with 10～50 μmol/L curcumin for 6～24 h and the growth inhibition rates of A2780 cancer cells were measured by MTT method. Cell apoptosis was inspected by flow cytometery (FCM) and acridine orange ethidium bromide fluorescent staining method. The protein levels of NF κB(P65) and Caspase 3 in cancer cells were observed by SP immunohistochemistry. Results: The growth inhibition rates of the cancer cells reached 62.05%～89.24%, with the peak of sub G1 appeared on DNA histogram in FCM. Partial cells presented the characteristic morphological changes of apoptosis under the fluorescent microscope;the apoptosis rates were 21.5%～33.5%. The NF κB (p65) expression was decreased while Caspase 3 expression was increased, which depended on the action time. Conclusions: Curcumin could significantly inhibit the growth of ovary cancer cells; inducing apoptosis through up regulating Caspase 3 and down regulating expression of NF κB was probably one of its molecular mechanisms.