不同处理下蛋青鸡血浆游离Leu比放射性活度的变化

Change regularity of special radioactivities of plasma free Leucine under four different treatments

在绝食、强饲无氮日粮(NFD)、CP20%(豆粕组)、CP20%(棉粕组)4种处理下,给蛋青鸡每公斤体重静脉一次注射1110MBq[3H]Leu,分别于注射后5min,30min,4h,24h,36h,48h翅静脉采血,测定各时间点去蛋白血浆游离Leu浓度和比放射性活度。结果表明,去蛋白血浆中游离Leu浓度绝食组和NFD组显著低于豆粕组和棉粕组(P<0.05);血浆游离Leu的比放射性活度(SRp),以NFD组与绝食组相近,且较高,棉粕组最低,但4种处理间无显著差异(P>0.05)。血浆游离Leu比放射性活度呈显著的指数衰变规律(P<0.05)。

Twenty four young hens (1.5 kg of body weight,BW) were randomly divded into 4 groups.Every group was diet free (FAS) or forcefed a nitrogenfree diet (NFD) or the diet with 20% crude protein in which soybean meal or cottonseed meal was the sole nitrogen source (30 g DM/kg BW).1 100 MBq3HLeucine/kg BW was intravenously injected into all birds just after forcefed or onfasting.Venous blood samples were taken at 5 min,30 min,4 h,24 h,36 h and 48 h after injection.Special radioactivities of nonprotein plasma at every timepoint are measured.The study showed that concentrations of free Leucine in plasma of FAS and NFD were significantly lower than that of CP 20% (soybean meal) and CP 20% (cottonseed meal) (\%P\%<0.05).Specific radioactivities of free Leucine in plasma in NFD and FAS were similar and higher and that of CP 20% (cottonseed meal) was the lowest,but there was no significant difference between four kinds of treatments (P>0.05).Specific radioactivity of free Leucine in plasma complied with significantly exponential function (P<0.05).