CD58及雌酮对妊娠早期山羊EML分泌IL-2及IL-4的影响

Effects of CD58 and estrone on the secretion of IL-2 and IL-4 by goat EML in early gestation

将妊娠17d山羊子宫内膜淋巴细胞(EML)放入含不同剂量PHA-P,CD58和雌酮的介质中进行体外培养,并分别用生物学方法和双抗体夹心ELISA法测定培养上清液中IL-2和IL-4的水平。结果表明,CD58和雌酮处理组EML分泌的IL-2水平低,与细胞对照组差异不显著,并显著低于PHA-P处理组。3个浓度CD58处理组IL-4分泌水平高达5.40～6.36ng/mL,高浓度雌酮处理组IL-4分泌量也达到4.77ng/mL,与细胞对照组差异均极显著,而且CD58能以剂量依赖方式促进EML对IL-4的分泌。

Endometrial lymphocytes (EMLs) of pregnant goat (17 d) were cultured in vitro respectively in media containing PHAP,CD58 and estrone at different doses.Then IL2 and IL4 in medium supernatants were measured respectively with biological method and doubleantibody ELISA.The results showed that EMLs treated with CD58 and estrone secreted IL2 at low level are insignificantly different from that of control cell group and significantly lower than that of EMLs treated with PHAP.The EMLs treated with CD58 in three concentrations dosedependently produced 5.40-6.36 ng/mL of IL4 and those with estrone at higher dose produced 4.77 ng/mL of IL4,all significantly higher than that of control cell group.