摘要
对采自云南各大烟区的烟草野火病菌株进行了分离、鉴定,用通过筛选致病力强的烟草野火病菌株为抗原,制备了烟草野火病菌特异性的抗血清,建立了一套快速检测烟草野火病菌的方法,制备了SPA-ELISA反应试剂盒,应用玻片凝集或协同凝集反应与SPA-ELISA反应试剂盒相结合的方法,使检测真正做到了简便、快速、灵敏、准确。
Strains of tobacco wildfire from Yunnan were isolated and identified.Pseudomonas syringae pv.tabaci specific antiserum was obtained through screening the strongvirulent strains as antigen.Straphylococal protein A ELISA (SPAELISA) kits were developed successfully and this was the first time to establish the rapid detecting method of the pathogen.Detection techniques become easier,rapider,more sensitive and accurate,by combining SPAELISA with agglutination test (slide agglutination and coagglutination).
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2002年第6期138-143,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
云南省烟草公司基金项目(98A55)