雌性大鼠动脉中雌激素受体的表达及意义

EXPRESSION AND MEANING OF ESTROGEN RECEPTOR IN THE ARTERY OF ADULT FEMALE RAT

目的 :从整体及细胞水平观察雌性成年大鼠动脉中雌激素受体的存在情况并探讨其存在意义。方法 :用放射配体结合分析法检测雌性成年大鼠主动脉及培养的血管内皮细胞中雌激素受体的含量。结果 :雌性成年大鼠动脉ER的结合容量及平均解离常数 (Kd值 )为 37.37± 7.85fmol/mgpro .和 2 .96± 0 .71× 10 -9mol;培养的血管内皮细胞中ER的结合容量及Kd值为 17.5 5± 1.2 5fmol/10 6细胞和 3.5 3± 1.2 5nmol。结论 :雌性大鼠主动脉壁及血管内皮细胞中存在雌激素受体 ,这为解释和探讨雌激素对心血管保护作用的机制提供了重要的分子基础。

Objective:To observe the expression of estrogen receptor in the artery of adult female rat from integer and cell level and also it's assess significance.Methods:The receptor binding assay was adopted to measure the estrogen receptors in the artery and endothelial cells of the rat.Results:The number and average affinity( Kd value) of ER in the artery of adult female rat are 37.37±7.85 fmol/mg pro and 2.96±0.71×10 -9 mol, and the number and affinity ( Kd value) of ER in the endothelial ceils of adult female rat are 7.55 ± 1.25 fmol/10 6 cells and 53 ±1.25nmol.Conclusion:The estrogen receptors exist in the artery and endothelial cells of female rats, which may contribute the molecular basis to explain the cardiovascular protective mechaism of estrogen.