KK-42对家蚕蛹血淋巴及卵巢蛋白质合成的影响

EFFECTS OF KK-42 ON PROTEIN BIOSYNTHESIS OF HEMOLYMPH AND OVARY OF PUPA IN BOMBYX MORI

研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。

In order to evaluate the acting mechanism of KK-42 on the ovarian development and protein biosynthesis , imidazole compound (KK-42) was applied to the pupae of the silkworms to investigate its effects on the protein biosynthesis of ovary and hemolymph. The results revealed that the increase of ovarian weight was inhibited markedly by KK-42. The protein bands of ovaries and hemolymph in pupa treated with KK-42 on gel electrophoresis had no difference compared with the control, but there was a slight change in the protein content of hemolymph and a little increase in the protein content of ovaries due to treatment of KK-42, while the protein content of hemolymph in the con-trol increased rapidly after pupation and reached peak on 3rd day, then declined rapidly and the increase of ovarian protein was identical with the decline of hemolymph protein. These suggested that not only protein biosynthesis in hemolymph but also transfer of protein from hemolymph to ovaries was inhibited by KK-42.