羊蛛网膜下植入APA微囊化牛嗜铬细胞脑脊液亮氨酸脑啡肽和儿茶酚胺含量的变化

Concentration change of catecholamine and leucine-enkephalin in sheep cerebrospinal fluid after APA microencapsulated bovine chromaffin cells transplantation to the spinal subarachnoid space

目的 :了解APA微囊化牛嗜铬细胞 (APA BCCs)蛛网膜下植入引起正常羊脑脊液中儿茶酚胺 (cate cholamine ,CA)和亮氨酸脑啡肽 (leucine enkephalin ,L EK)含量的变化 ,分析APA BCCs移植镇痛的可能机制及APA微囊的免疫保护效应。方法 :以手术方法分别将APA BCCs、BCCs或空微囊植入正常羊脊髓蛛网膜下 ,测定移植前后不同时间羊脑脊液中去甲肾上腺素 (norepinephrine,NE)、肾上腺素 (epinephrine ,E)和L EK的含量。结果 :与移植前羊脑脊液中NE、E和L EK的水平相比 ,空微囊植入未能升高 ;BCCs植入可在 7d内升高 ;APA BCCs植入可明显升高超过 2 8d(P <0 0 5 )。结论 :蛛网膜下植入BCCs能显著升高宿主羊脑脊液中CA和L EK的水平 ,这可能与BCCs蛛网膜下移植的镇痛效应有关 ;APA微囊包裹有利于BCCs在异种动物体内较长期地分泌镇痛物质。

Objective:To observe the change of catecholamine (CA) and leucine-enkephalin(L-EK) concentration in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells transplantation to the spinal subarachnoid space, to analyze the analgesic mechanism of transplantation and the immuno-protecting effect of APA-microcapsulation. Methods: To check concentration of norepinephrine(NE), epinephrine(E) and leucine-enkephalin(L-EK) in sheep cerebrospinal fluid after APA Microencapsulated Bovine Chromaffin Cells(APA-BCCs), BCCs and hollow microcapsule transplantation to the spinal subarachnoid space. Results: the concentration of E, NE and L-EK didn't increased after transplantation of hollow microcapsule,increased after BCCs transplantation within 7 days and APA-BCCs transplantation within 28 days. Conclusion: BCCs transplantation to the spinal subarachnoid space could improve the concentration of CA and L-EK in host cerebrospinal fluid, the improvement may be related to analgesic effect, and APA-microencapsulation is good for lasting secretion of BCCs.