LRP16编码蛋白的信息学分析及亚细胞定位研究

Bioinformatic analysis and subcellular distribution of LRP16 protein

目的 :探讨LRP16基因编码蛋白的基本生物学特征及其在真核细胞中的分布。方法 :以Internet为操作平台、以国际公共生物数据库为实验材料、应用生物学软件为研究工具对LRP16编码蛋白进行了全方位的生物信息学分析。在此基础上 ,将该基因的编码序列重组到pEGFP N1真核表达中 ,融合在绿色荧光蛋白 (GFP)的N端转染HeLa细胞 ,激光共聚焦显微镜观察荧光分布。结果 :LRP16编码产物主要与一些RNA病毒产生的与核酸结合的非结构蛋白有较高相似性 ,含有与功能未知的Motif:Hismacro、DUF2 7、A1pp相似的结构域。激光共聚焦显微镜扫描发现GFP分布于细胞核内。结论 :LRP16基因编码一种起源古老、进化缓慢的核蛋白因子 。

Objective:To explore the basic biologically characteristics and the intracellular distribution of LRP16 protein cloned by us. Methods: The primarily structural sequence of LRP16 protein was thoroughly analysed using public databases provided by Internet and biological softwares. On the basis of bioinformatical hint, LRP16 was recombined in pEGFP-N1 vector, inserting in the N-termini of GFP(green fluorescence protein),and then transferred into HeLa cell line. Fluorescent signal was detected using confocal.Results: LRP16 protein has relatively higher similarity with some non-structural proteins coded by RNA virus, which are nucleic acid binding proteins. In addition,LRP16 has similar motif to the function-unknown Hismacro,DUF27 and A1pp.The green fluorescence was inspired from cellular nucleolus. Conclusion:LRP16 protein is a nuclear factor, which originated prior to the appearance of eukaryotes, and its evolution velocity is relatively slow and its motif has retained the basic function of ancestral gene. The motif feature provide a clue that LRP16 protein maybe play an important function in cellular multiple behavior.