胃腺癌nm23表达与肿瘤细胞增殖周期的关系及意义

Relationship and siginificance of nm23 expression and the cell cycle of gastric adenocarcinoma

目的 研究胃腺癌中肿瘤转移抑制基因nm2 3与肿瘤细胞增殖周期因子 (PCNA、S期细胞比 )的关系。方法 采用免疫组化SP法检测 6 5例胃腺癌标本中nm2 3、PCNA的表达 ,同时应用Feulgen染色方法及计算机图象分析技术对其S期细胞比进行检测。结果 nm2 3、PCNA、S期细胞比均与胃腺癌淋巴结转移有关 ;nm2 3表达与PCNA分级、S期细胞比呈负相关。结论 nm2 3能抑制胃腺癌的转移 ,其抑癌机理可能与抑制肿瘤细胞增殖周期有关。

Objective To explore the relationship and significance between nm23 and cell cycle factors (PCNA, SPF) in 65 cases of primary gastric adenocarcinoma. Methods The expressions of nm23 ?PCNA and SPF (S\|phase fraction ) were investigated by using SP immunohistochemical staining ,Feulgen DNA histochemical staining and image analysis. Results All their expressions were correlated with tumor metastasis;there is an inversing association between nm23 and PCNA?SPF. Conclusion nm23 gene can suppress the metastasis of gastric adenocarcinoma. The possible mechanism is the nm23 gene can suppress the tumor cellular proliferative cycle