甲状腺乳头状癌中CD44v6和p16基因表达的原位杂交研究

Expression of CD44v6 and p16 gene in papillary thyroid carcinoma studied by in situ hybridization

目的 探讨CD4 4v6和 p16基因表达与甲状腺乳头状癌 (PTC)侵袭转移的关系。 方法 应用原位杂交方法 ,检测 4 6例PTC组织中CD4 4v6和 p16的mRNA表达。 结果 PTC组织中CD4 4v6和 p16的mRNA表达阳性率分别为 76 .1%和 6 0 .9% ,均与PTC侵袭转移相关 (P <0 .0 5 ) ;CD4 4v6mRNA与 p16mRNA表达呈负相关 (r = 0 .36 ,P <0 .0 0 5 )。结论 CD4 4v6和 p16基因表达可作为判断PTC预后的参考指标。

Objective To investigate the correlation of CD44v6 and p16 gene expression with the potential of invasion and metastasis in papillary thyroid carcinoma(PTC).Methods In situ hybridization method was used to detect CD44v6 mRNA and p16 mRNA expression in 46 cases of PTC.Results The positive rates of CD44v6 mRNA and p16 mRNA in PTC were 76.1% and 60.9% respectively.There was a positive correlation between CD44v6 mRNA expression and tumor invasion and metastasic potential in PTC( P <0.05),and a reverse correlation between p16 mRNA expression ( P <0.05).Moreover,there was a reverse correlation between the CD44v6 mRNA and p16 mRNA expression in PTC( r =\|0.36, P <0.005).Conclusion CD44v6 mRNA and p16 mRNA may be prognostic indicators in PTC.