食管癌Fhit蛋白表达丢失的临床病理研究

Loss of fragile histidine triad protein expression in esophageal carcinoma

目的 探讨食管癌Fhit蛋白表达与临床病理的关系。方法 采用免疫组化方法 ,检测 5 3例食管癌Fhit表达并分析其与组织学分级、临床分期的关系。结果 癌组织Fhit表达为 2 3/ 5 3例 (43.4 % ) ,而癌旁非肿瘤粘膜为 4 7/ 5 3例 (88.7% ) ,相差有非常显著性 (P <0 .0 0 1)。Fhit蛋白表达癌在癌组织学分级中的分布为Ⅰ级癌 5 5 .6 % (5 / 9) ,Ⅱ级癌 4 0 .6 % (13/ 32 ) ,Ⅲ级癌 4 1.7% (5 / 12 ) ,相差无显著性 (P >0 .0 5 )。Fhit蛋白表达癌在临床分期中的分布为Ⅰ期癌 77.8% (7/ 9) ,Ⅱ期癌 4 2 .9% (15 / 35 ) ,Ⅲ期癌 11.1% (1/ 9) ,相差有非常显著性 (P <0 .0 0 1)。

Objective To investigate the expression of fragile histidine triad protein, Fhit and the possible relationship between Fhit expression and clinicopathological indices in esophageal carcinoma.Methods Detecting Fhit protein expression in 53cases of esophageal carcinoma and analysing its relationship to histological grade and clinical stage by immunohistochemical method. Results The expression of Fhit protein was positive in 23/53 (43.4%) cases of cancer tissue and in 47/53 (88.7%) cases of adjacent non\|tumoral tissue. There was a significant difference in the expression of Fhit protein between cancer and adjacent non\|tumoral tissue( P <0.001). It was showed that Fhit protein was expressed in 5/9 (55.6%), 13/32 (40.6%) and 5/12 (41.7%) cases of carcinomas in grade Ⅰ, Ⅱand Ⅲ, respectively and no difference was found between the grades of tumor ( P >0.05). The significant difference in the expression of Fhit was found between cancers on clinical stage Ⅰ(7/9, 77.8%), Ⅱ(15/35, 42.9%) and Ⅲ(1/9,11.1%), respectively ( P <0.001). Conclusion Loss of Fhit protein expression may be associated with invasion and metastasis in the esophageal carcinoma.