非有机溶剂液-固萃取体系提取血红蛋白研究

A Study of Abstraction of Hemoglobin by Non-organic Solvent Liquid-solid Extraction System

用聚乙二醇 ( PEG80 0 0 )修饰物混合吐温 80 -磷酸钾盐液 -固萃取体系从血液中直接提取血红蛋白 ,考察了 PEG80 0 0修饰物、磷酸钾盐和吐温 80的浓度及酸度和温度等因素对血红蛋白分离纯化的影响 .结果表明 ,采用该体系的一次固相萃取率大于 99% ,一次反萃取率达到 75 % .所得到的血红蛋白干粉的纯度为99% .该方法不使用有机溶剂 ,无毒性 ,对蛋白质具有稳定和保护作用 ,易于放大规模使用 .

Polymer-salt-aqueous liquid-solid extraction system is newly developed in 1990′s. The system has been successfully used for extraction of metal ions and bioactive substances such as malic dehydrogenase. The separation and extraction of hemoglobin by modified PEG 8000 mixed with Tween 80 and aqueous K 3PO 4 system is seldom reported. The easy phase formation by this method can make solid and liquid phases separated without any treatment, so that the imperfection of liquid biphasic technology can be eliminated. The method mentioned in this paper has the features of high capacity, cost effectiveness, convenience. Meanwhile the polymer and salt comprising the extraction system can stabilize and protect the bioactive substances. So the new technology is very suitable for the practical use in large scale. In the research, extraction efficiency from the solid phase is >99% and reversed extraction efficiency can reach 75%. The purity of hemoglobin is 99% after reversed extraction. Highly purified hemoglobin powder can be produced through dialysis and freeze drying treatment.