胃癌中P16、Rb蛋白表达分析

Expression of P16 and Rb Proteins in Gastric Carcinoma

目的 研究p16和Rb基因表达产物P16、Rb蛋白与胃癌发生发展的关系及分析胃癌组织中p16、Rb基因表达的相互关系。方法 运用链霉菌抗生物素蛋白 -过氧化物酶 (S -P)免疫组织化学方法检测胃癌、癌前病变及正常胃粘膜组织中P16、RB蛋白的表达。结果 P16、Rb蛋白表达阳性率分别为 :正常胃粘膜 96 2 5 %(77/ 80 )、98 75 % (79/ 80 ) ,不典型增生 92 0 0 % (46 / 5 0 )、80 0 0 % (40 / 5 0 ) ,胃癌 4 7 5 4% (5 8/ 12 2 )、5 9 84 % (73/ 12 2 ) ,胃癌组中P16、Rb蛋白表达低于正常胃粘膜及不典型增生 (P <0 0 5 ) ;胃癌中P16、Rb蛋白的表达的相互关系 :P16、Rb蛋白均为阳性 15 5 6 % (14 / 90 ) ,P16、Rb蛋白均为阴性 7 78% (7/ 90 ) ,P16蛋白阳性、Rb蛋白阴性 33 33%(30 / 90 ) ,P16蛋白阴性、Rb蛋白阳性 4 3 33% (39/ 90 ) ,P16、Rb蛋白表达呈负相关 (P <0 0 5 )。结论 P16、Rb蛋白表达缺失与胃癌的发生发展有关 ;胃癌组织中P16蛋白与Rb蛋白表达呈负相关关系。

Objective It was designed to investigate the relationship between the productions of p16 and Rb genes expression and gastric carcinogenesis and evaluate the correlation between the expression of p16 and Rb genes in gastric carcinoma.Methods The expression of P16 and Rb proteins was examined in gastric carcinoma and precancerous lesion and normal gastric mucosa by streptavidin-peroxidase immunohistochemical method (S-P).Results The positive rates of P16 and Rb proteins expression showed as below: There were 96 25%(77/80) and 98 75%(79/80) in normal gastric mucosa, 92 00%(46/50) and 80 00%(40/50) in atypical hyperplasia gastric mucosa and 47 54%(58/122) and 59 84%(73/122) in gastric carcinoma respectively. The positive rates of P16 and Rb proteins expression in gastric carcinoma were significantly lower than that in normal gastric mucosa and atypical hyperplasia gastric mucosa (P<0 05). The relationship of expression between P16 and Rb proteins in gastric carcinoma:the positive rate of P16 and Rb proteins was 15 56%(14/90).The negative rate of P16 and Rb proteins was 7 78%(7/90).The P16 protein positive but Rb protein negative was 33 33%(30/90). The P16 protein negative but the Rb protein positive rate was 43 33%(39/90).Inverse correlation of P16 and Rb proteins expression was found in gastric carcinoma (P<0 05).Conclusions The expression loss of P16 and Rb proteins is related to carcinogenesis of gastric carcinoma. There is inverse correlation of P16 and Rb proteins expression in gastric carcinoma.