甘油对离子注入动物细胞的保护效应

Protective Effects of Glycerine on Mammalian Cells in Ion Implantation

提出一种方法,用低浓度甘油、适度预处理时间,可有效提高哺乳动物细胞对真空的耐受力,从而为开展离子注入动物细胞的研究探索出一条新路.离子注入生物效应的发现开辟了辐射生物学的一个新领域———低能重离子生物学.由于离子注入要在真空中进行,迄今这一领域研究的对象主要是对真空有一定耐受性的植物种子和微生物。

The discovery of bio effects induced by ion implantation has opened a novel branch in the field of radiation biology——the low energy heavy ion biology. The experimental samples in the field have, however, been within the limits of plants and microbes because of the sample's vacuum resistant ability. The mutation studying of mammalian cells with ion implantation hasn't been reported in the past years.The main reason for that is that it is difficult to keep mammalian cells alive in vacuum during ion implantation. In the present study, the tolerance of mammalian cells in vacuum was enhanced efficiently by pre treatment with glycerin at low concentration and proper treatment time, which provides a new way for the study on the biological effects of ion beam implantation in the mammalian cells.