半知菌曲霉族真菌胞外多糖的研究Ⅱ:爪哇正青霉胞外多糖化学结构的研究

A study on extracellular polysaccharides from Aspergileae of fungi imperfecti Ⅱ: chemical structure of extracellular polysaccharides from Eupenicillium javanicum

经形态鉴定和 18srRNA基因组序列分析 ,编号Ym31794菌株的分类学地位属于半知菌类丛梗孢科曲霉族青霉属丝状真菌 ,种名爪哇正青霉 (Eupenicilliumjavanicum) .该菌发酵液经离心弃菌体 ,除脂类及乙醇分级提取 ,分离、纯化的胞外多糖相对分子量为 2 .6× 10 4,糖的质量分数为 89% .真菌胞外多糖经红外光谱(IR) ,簿层层析 (TLC) ,色质联用 (GC -MS) ,核磁共振 (13 C -NMR ,1H -NMR)等分析可知爪哇正青霉菌胞外多糖为 1- 6连接的葡萄糖和 1- 2连接半乳糖构成主干 ,侧链由 1- 3连接的甘露糖和 1- 2连接的葡萄糖构成 ,结构应为吡喃糖 .

A strain YM31794,producing extracellular polysaccharide,was identified,using the methods of morphology and 18s rDNA sequencing analysis,as Eupenicillium javanicum Thom.The chemical structure of the extracellular polysaccharide from this strain was analysed through the reaction of methyl,Smith's degradation,thin layer chromatography,GC MS chromatography,IR spectrum, 13 C NMR and 1H NMR.The result indicate that this polysaccharide is a pyranose which is composed of the 1,6 bond linked glucose,1,2 bond linked galactose and 1,3 bond linked mannose ,and 1,2 bond linked glucose on its side chain.