鼠伤寒沙门氏菌SOD不同途径免疫小鼠的效果观察

Diversity of antibody-mediated immunity with salmonella typhimurium SOD in mouse vaccinated by different routes

目的 观察用鼠伤寒沙门氏菌SOD作为抗原 ,由不同途径免疫小鼠的抗体产生情况。方法 Balb c小鼠按免疫途径分为 4组———腹腔注射、灌胃、滴鼻与肌注组 ,各组抗原量相同。于末次免疫后第 1 0、1 5、2 0和 30天采血 ,第 30天时杀鼠取肠液 ,用间接ELISA法检测IgA及IgG效价。结果 黏膜外途径免疫小鼠以IgG应答为主 ;黏膜途径免疫则可建立较好的局部粘膜应答 ,其中滴鼻组还可引起较好的全身性应答。

Objective To explore the possibility whether the mouse could be protected from infection by local anti SOD antibody. Because SOD is one of bacterial pathogenesis and the most infections begin at a mucosal site.Methods Mice were divided into 4 groups, and then they were immunized with salmonella typhimurium SOD by different routes ( peritoneal, gastric, nasal and muscular) respectively.After that the titers of anti SOD IgG and IgA in serum or in gut juice were detected. The titers were assayed by indirect ELISA test.Results The results suggested that serum IgG was the major product when the mice were vaccinated by peritoneal or muscular route, and the local immune globulins was better in mucosal immunized groups ( especially in the nasal immune group )during the period we observed.Conclusions SOD not only could cause local immune response, but also system response, so it is possible to draw a conclusion that the mouse could acquire special immunity to respond against the Salmonella typhimurium SOD if they were immunized by mucosal routes.