IL-18对系统性红斑狼疮患者淋巴细胞凋亡和P53蛋白表达的影响

Lymphocyte apoptosis and P53 expression in peripheral blood lymphocytes of patients with systemic lupus erythematosus stimulated by IL-18

目的 研究系统性红斑狼疮 (SLE)患者外周血淋巴细胞在体外IL 1 8刺激培养下细胞凋亡及P53蛋白表达情况。方法 AnnexinV联合PI染色定量法及免疫荧光染色法 ,分析了 44例SLE患者和 30例正常人外周淋巴细胞在体外IL 1 8刺激培养后凋亡发生率 ,凋亡相关基因P53蛋白的表达以及淋巴细胞凋亡发生与疾病活动性的相关性。结果 在IL 1 8刺激培养作用下 ,活动期SLE淋巴细胞凋亡发生率较正常人显著增高 (P <0 .0 1 ) ,而静止期则无明显变化 (P >0 .0 5)。P53蛋白表达在活动期SLE较正常人显著性下降 (P <0 .0 1 ) ,静止期无明显变化 (P >0 .0 5)。P53的表达与疾病活动指数SLEDAI之间有明显的相关性 (P <0 .0 1 )。结论 IL 1 8可引起SLE患者PBL凋亡率的增高 ,表明IL 1

Objective To study the change of lymphocyte apoptosis and P53 protein in the peripheral blood of SLE patients. Methods Apoptosis and P53 protein in lymphocyte were investigated in 44 SLE patients and 30 normal people by using annexin V united PI staining method and immunofluorescence staining method. Results The percentage of lymphocyte apoptosis from active SLE was significantly higher than that of normal control ( P< 0.01). No significant difference of apoptosis was found in active SLE as compared with control ( P> 0.05). Expression of P53 protein in lymphocyte from active SLE was significantly lower than that of control ( P< 0.01). No significant diffe rence in the expression of P53 protein was found in active SLE as compared with that of control ( P> 0.05). Expression of P53 protein in lymphocyte from SLE patients showed high correlation with SLEDAI, dsDNA and C3 ( P< 0.01). Conclusion The percentage of PBMC apoptosis from active SLE patients stimulated by IL 18 was increased, suggesting that IL 18 could influence the apoptosis in vivo and immune mechanism of apoptosis.