遍地金提取物对大鼠脑纹状体突触体[^3H]－多巴胺重摄取抑制试验

Effect of Hypericum Wightianum extracts on synapse reuptake of dopamine in the striatum of rat brain

目的:评价遍地金提取物对大鼠脑纹状体突触体[3H]-多巴胺重摄取的抑制作用。方法:通过大鼠脑纹状体突触体[3H]-多巴胺重摄取抑制实验,测定其重摄取量cmp及抑制百分率,以评价待试物对多巴胺重摄取的抑制活性。结果:遍地金组平均抑制百分率为65％,IC50=113.25mg·ml-1;St.John's Wort组平均抑制百分率为61％,IC50=28.29mg·ml-1。结论:遍地金提取物与在欧美上市的药物 St.john's Wort均抑制大鼠脑纹状体突触体[3H]-多巴胺重摄取,二者差异无统计学意义。

OBJECTIVE: To study effect of hypericum wightianum extracts on synapse reuptake of dopamine in rat brain striatum. METHODS:The inhibition experiment of reuptaking [3H] -dopamine on brain striatum synapse of rat has been adopted. The activity was evaluated by assaying quantity of reuptaking(cmp) and the inhibition percentage. RESULTS:The average inhibition percentage and IC50 of Hypericum Wightianum extract group,are respectively 65% and 113. 25 mg/ml and for St. Johns 61% and 28. 29mg-ml-1 and for Wort group are. CONCLUSION :Hypericum Wightianum extract group and antidepressant preparation St. John's Wort group both showed inhibition activity on brain striatum synapse reuptaking dopamine and have no statistics significance between the two groups.