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狼疮肾炎中识别新抗原的抗中性粒细胞胞质抗体及其与光过敏和口腔溃疡的关系 被引量:3

Antigens of antineutrophil cytoplasmic autoantibodies in recognizing a novel neutrophil granule antigen in lupus nephritis and its association with photosensitivity and oral ulceration
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摘要 目的 寻找狼疮肾炎 (LN)患者中抗中性粒细胞胞质抗体 (ANCA)的未知靶抗原 ,并分析其相应抗体与临床和病理损害的关系。方法 收集 72例有完整临床病理资料的狼疮肾炎患者的血清 ,分离健康人外周血中性粒细胞的初级颗粒和次级颗粒制备成酸性可溶性颗粒蛋白抗原液 ,以此为抗原在非还原状态下进行Western blot分析 ,检测狼疮肾炎患者血清中的ANCA。结果 部分血清可识别一些蛋白条带 ,其中 1 4 / 72 (1 9 4% )例识别分子量为 690 0 0的蛋白条带 ,后者不同于ANCA其他已知的靶抗原如组织蛋白酶G和乳铁蛋白等 ;1 0 / 72 (1 3 9% )例识别 55 0 0 0的蛋白条带 ,后者可能是杀菌 /通透性增高蛋白。进一步的研究发现 ,在抗 690 0 0蛋白抗体阳性的病人中 ,光过敏和口腔溃疡的发生率均显著高于抗 690 0 0蛋白抗体阴性者 (分别为 57 1 %、1 2 1 % ,P <0 0 0 5 ;50 0 %、1 7 2 % ,P <0 0 5)。结论 中性粒细胞混合颗粒中的 690 0 0蛋白可能为狼疮肾炎ANCA新的特异性靶抗原 ,抗 690 0 0蛋白抗体可能与狼疮肾炎患者光过敏和口腔溃疡相关。 Objective To investigate whether there are some unknown target antigens of antineutrophil cytoplasmic autoantibodies (ANCA) in patients with lupus nephritis (LN) and the prevalence and the clinical significance of these unknown target antigens Methods Seum was collected from 72 renal biopsy proven lupus nephritis (LN) patients Mixed neutrophil granules were separated from normal human peripheral neutrophils and soluble acid extracts in non reducing conditions were used as antigens in Western blot analysis to detect ANCA in the serum sample of LN patients Results Serum from seme LN patients could blot a few bands Interestingly, serum from 14/72 (19 4%) of the patients recognized a novel 69 000 protein band and that from 10/72 (13 9%) sera recognized a 55 000 protein band that might be the bactericidal/permeability increasing protein (BPI) The 69 000 target antigen was different from the known target ANCA antigens such as cathepsin G and lactoferrin Further study revealed that the percentages of patients with photosensitivity and oral ulcer in anti 69 000 autoantibody positive group were significantly higher than those in the anti 69 000 autoantibody negative group (57 1% vs 12 1%, P <0 005, and 50 0% vs 17 2%, P <0 05, respectively) Conclusions A novel 69 000 protein in human neutrophil granules is identified as a target antigen of ANCA in LN patients The anti 69 000 autoantibodies may be associated with photosensitivity and oral ulcer in LN patients
出处 《中华内科杂志》 CAS CSCD 北大核心 2002年第11期766-768,共3页 Chinese Journal of Internal Medicine
基金 国家教委霍英东青年教师基金 教育部优秀青年教师基金资助
关键词 狼疮肾炎 抗原 抗中性粒细胞胞质抗体 光过敏 口腔溃疡 Lupus nephritis Antibodies, antineutrophil cytoplasmic Autoantigens
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  • 1蒋克春,风湿病学杂志,1996年,1期,130页

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