摘要
目的 探讨阿霉素耐药乳腺癌细胞系MCF 7/ADM的ABC家族基因的表达及其与敏感细胞系MCF 7/S的差异 ,观察中药雄黄对ABC膜转运蛋白超家族中各基因 (mdr 1、mrp、bcrp)表达的影响。 方法 应用RT PCR检测ABC家族基因的转录表达 ,MTT法检测耐药性改变。 结果 ABC家族基因在MCF 7/ADM细胞中均过表达 ,而在MCF 7/S细胞中均未见表达 ;雄黄可下调ABC家族基因的mRNA水平 ,降低化疗药物阿霉素 (ADM)对MCF 7/ADM细胞的IC50 。 结论 乳腺癌MCF 7/ADM细胞获得ADM抗药性 ,与ABC家族基因过表达有密切关系 ;雄黄可部分逆转MCF 7/ADM细胞对ADM的抗药性。
Objective To study the expression of ABC gene family of MCF 7/ADM cells as compared to parental cell line MCF 7/S, and its reversion by realgar of the expressed genes. Methods Expression of ABC family genes was examined by RT PCR, and cell growth by MTT test. Results The expression of ABC gene family was positive in MCF 7/ADM cells compared to MCF 7/S cells; Realgar can down regulate the expression ABC gene family in MCF 7/ADM cells; the IC 50 of ADM to MCF 7/ADM cells was decreased.Conclusion ABC gene family was responsible for the induced resistance of MCF 7/ADM cells to ADM; Realgar is able to reverse partly the multidrug resistance of the human breast cancer cell line MCF 7/ADM. [
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第6期623-626,共4页
Acta Anatomica Sinica
