神经损伤与再生中神经元GAP-43mRNA表达的原位杂交研究

In situ HYBRIZATION STUDY ON GAP-43mRNA EXPRESSION DURING NERVE INJURE

目的 研究周围神经损伤过程中生长相关蛋白 (GAP 4 3)mRNA表达的变化规律。 方法 建立大鼠坐骨神经中段钳夹损伤模型 ,用原位杂交技术对大鼠的腰髓和背根神经节的GAP 4 3mRNA表达进行观察。 结果 大鼠坐骨神经损伤 2d后 ,腰髓腹角运动神经元和背根节感觉神经元可检测到GAP 4 3mRNA杂交信号 ;术后 4、7和 14d明显增强 ;术后 30d减弱 ,6 0d已恢复正常。 结论 周围神经损伤诱导神经元胞体GAP 4 3mRNA表达显著增强 ,表明GAP 4 3在神经再生过程中起重要作用。

Objective To study GAP\|43mRNA expression during nerve injure and regeneration. Methods Rat sciatic nerve was crushed then, in situ hybridization technique was used to explore GAP\|43mRNA expressions in spinal cord and dorsal root ganglion (DRG). Results The neurons of spinal cord and DRG were detected to have GAP\|43 hybridization sinal by 2 days after sciatic nerve lesion. At later times(4,7 and 14 days postsurgery) the anterior horn motor neurons and DRG cells showed an increase in the number of GAP\|43mRNA positive neurons, followed by a significant rise in their content of GAP\|43mRNA. The number of GAP\|43mRNA positive neurons was decreased by 30 days postinjure, and was nearly nomal 60 days postinjure.Conclusion GAP\|43mRNA expression was increased during peripheral nerve injure and regeneration. The study showed that GAP\|43 may play a key role in nerve regeneration. [