摘要
目的 分离和纯化猪的精原细胞 ,从而对与人类具有高同源性的猪的精子发生进行研究。 方法 酶消化法制备 2月龄未成熟长白猪睾丸组织的单细胞悬液 ,以 2 %～ 4 %牛血清白蛋白 (BSA)连续梯度作为分离介质 ,采用重力沉降法并结合细胞贴壁培养的方法分离精原细胞。 结果 重力沉降法分离细胞后所获精原细胞纯度为 91% ,进一步经过贴壁培养纯化后 ,精原细胞纯度达到 94 2 %。 结论 该方法方便、快捷 ,分离效果好 ,能满足在分子水平研究精原细胞的需要。
Objective To isolate and purify spermatogonia from 2 month\|old Changbai boar testis. Methods After enzymatic digestions of boar testis, the suspension passed the 2%\|4% BSA continuous gradient medium in a gravity unit by velocity sedimentation. Then the spermatogonia were further purified by attachment culture. Results The purity of spermatogonia was 91% after velocity sedimentation separation.94% of spermatogonia was obtained after further pruification by attachment culture.Conclusion The method of isolating spermatogonia is convenient, fast and efficient. It could meet the needs for molecular studies on spermatogonia. [
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第6期662-664,共3页
Acta Anatomica Sinica
基金
国家攀登计划资助项目 [G19990 5 5 9(0 1) ]
