摘要
本实验采用显微镜和722分光光度计分别观察测定了冻融蟾蜍脑组织碱性磷酯酶(AKPase)活性,及五味子合剂的保护效应,结果表明,药物复温组血浆脂质过氧化物(LPO)和红细胞的malondialdehyde(mda)含量显著低于对照组(P<0.01);全血谷光甘肽过氧化物酶(GSH-Px)活性显著高于对照组(P<0.01);脑组织AKPase活性显著高于对照组呈强阳性。提示:五味子合剂具有抗脂质过氧化,保护GSH-Px酶活性,对冻融脑组织能间接提高机体清除超氧自由基和羟自由基的能力。这为组织器官的保存和移植提供了理论依据。
The determination of the AKPase of the freezing thawing toad, the whole body GSH-Pxase, LPO of the plamsa and MDA of brain cell was made using Olympus microscopic and 722-uv-speetrum(made in Japan). The results showed that LPO and MDA levels in experimental group were much lower and the activity of GSH-Pxase was much higher and (P<0.01) the AKPase of brain was markedly positive compared with the control group. In conclusion, the mixture of Wuweizi possesses the function of anti-poeroxadation of lipid and the protection of the activity of GSH-pxase, indirectly improving the ability of clearing the body of the peroxygen free redial and hydroxyl free redial the body.
出处
《黑龙江医药科学》
2002年第6期13-13,共1页
Heilongjiang Medicine and Pharmacy
