中国独龙族15个短串联重复序列基因座遗传多态性研究

Genetic polymorphism of fifteen short tandem repeat loci in Chinese Drungs

目的 研究我国独龙族短串联重复序列 (short tandem repeat,STR)遗传多态性。方法 应用 STR复合扩增、基因扫描、基因分型方法 ,调查了 6 7名中国独龙族无关个体 15个 STR基因座的等位基因及其基因型分布情况。结果 共检出 144个 STR等位基因和 2 6 0种基因型 ,其频率分布在 0 .0 0 77～0 .7846和 0 .0 15 4～ 0 .6 46 2之间 ,杂合度为 0 .372 3～ 0 .86 39,个体识别率为 0 .5 5 6 7～ 0 .95 48,非父排除率为 0 .2 738～ 0 .835 8,多态信息量为 0 .34 6 1～ 0 .845 6 ,累计个体识别力和非父排除率分别达 0 .99999998和0 .99999894。结论 揭示了我国独龙族 15个 STR基因座遗传结构特征 ,为人类学、法医学等研究提供了数据 ,也为进一步研究中华民族 STR遗传多态性奠定了基础。

Objective To study the short tandem repeat (STR) polymorphism in Chinese Drungs (Tulungs). Methods The genetic distributions of fifteen STR loci were investigated with the use of coamplification, genescan and genotype from 67 Drungs. Results There were 144 STR alleles in Drung nationality, with their frequencies ranging from 0.0077 to 0.7846, heterozygosity(H)0.3723-0.8639, discrimination power(DP) 0.5567-0.9548, probability of paternity exclusion(EPP) 0.2738-0.8358, polymorphism information content (PIC) 0 3461-0 8456,the accumulative DP 0 99999998 and EPP 0 99999894.Conclusion The results of this study on the STR polymorphism in Chinese Drungs could be used as a basis for the genetic structure of Chinese ethnic groups and also be of significant application in anthropology and forensic science.