豚鼠耳蜗微血管内皮细胞的跨内皮细胞电阻及对牛血清白蛋白的通透性

Transendothelium electric resistance and permeability on bovine serum album of cochlear microvascular endothelial cells from guinea pig

目的 :研究豚鼠耳蜗微血管内皮细胞跨细胞电阻的动态变化及峰值大小 ,以及细胞单层对12 5I 牛血清白蛋白的通透性。方法 :①用小池技术建立起该内皮细胞通透性的体外模型 ;②研究该体外模型跨细胞电阻及对12 5I 牛血清白蛋白的通透性。结果 :①耳蜗内皮细胞在培养增殖过程中其跨细胞电阻呈动态变化过程 ,以 5×10 4/cm2 的细胞密度接种时 ,7d左右电阻到达峰值 ,其跨细胞电阻峰值大小为 (118.9± 18.5 )Ω/cm2 ;②体外模型中加入12 5I 牛血清白蛋白后 ,贝克 时间变化图呈抛物线型上升曲线 ,90min内几乎呈直线。结论 :跨细胞电阻及对12 5I 牛血清白蛋白变化曲线反映了体外耳蜗微血管内皮细胞的通透性特征。

Objective:To study the dynamic changes and the peak phases of transendothelial electric resistance (TEER) during the growth of cochlear microvascular endothelial cells (CMEC) in guinea pigs and the monolayer endothelial cells' permeability on radioiodinated 125 I bovine serum album (RISA). Method: ①Establish a model in vitro for the permeability of CMEC using small pool technique. ② Research the model's TEER and its permeability on RISA. Result: ① The TEER changed dynamically and reached the peak about seven days after inoculation of culture cells at 5×10 4/cm 2 level during the CMEC proliferated. The peak values of TEER was 118.9 ± 18.5Ω /cm 2; ②After RISA administration, the Bake time curve took on an ascending parabola, while within 90 min appeared like a straight line. Conclusion: The TEER and the Varity curve on RISA reveals the permeability of CMEC in vitro.