摘要
以新一代HSV Amplicon做载体 ,探讨在体外培养的小鼠耳蜗螺旋神经节细胞 (SGNC)中转导神经营养因子 3(NT 3)的表达和对听觉神经系统的神经元发育和存活的影响。实验构建了表达NT 3的HSV Amplicon重组体 ,经过无辅助病毒的包装细胞系包装后 ,感染体外培养的小鼠SGNC。结果显示HSV Amplicon转导的NT 3表达 ,能够刺激SGNC分泌较高水平的NT 3;SGNC的神经突起增长 ;耐受顺铂 (DDP)的毒性作用。其作用的机制可能是抑制Caspase 3前体的活化 ,减少了SGNC进入凋亡。
We constructed the HSV Amplicon vector, which transduced the neurotrophin 3(NT 3)/myc into the inner ear cell cultures. Helper virus free amplicon stocks were assessed in vitro for their capacity to induce the expression of NT 3 in cultured inner ear cells. HSVnt 3myc/lac mediated transduction of cultured cells at a MOI of 1 resulted in the production of NT 3 in a 48. The transduction of NT 3 harbored significantly greater numbers of surviving SGNC, suggesting that the over expression of neurotrophin 3 by HSV Amplicon attenuated the mouse SGNC from cisplatin induced damage through prohibiting caspase 3 activation. For the treatment of deaf patients with cochlear prostheses, the neurotrophin intervention may have clinical potentials in terms of enhancement of responsiveness in afferent peripheral nerve.
出处
《基础医学与临床》
CSCD
北大核心
2002年第5期409-412,F003,共5页
Basic and Clinical Medicine
基金
国家教委留学人员启动基金 (2 0 0 2 )
