中药金叶败毒颗粒抑制人巨细胞病毒感染丝裂素活化蛋白激酶p38信号通路的研究

Study on Effect of Jingye Baidu Granule on Inhibition of Mitosin Activating Protein Kinase (p38) Signaling Pathway with Human Cytomegalovirus Infection

目的 :研究金叶败毒颗粒 (简称中药 )对人巨细胞病毒 (HCMV)感染丝裂素活化蛋白激酶(MAPK) p38信号通路的抑制作用 ,并探讨其治疗HCMV感染的分子机制。 方法 :应用原位杂交和免疫组化方法分别检测中药和更昔洛韦 (GCV)干预HCMV感染的细胞 p38mRNA及 pRb蛋白表达水平 ,观察两种药物对HCMV感染人胚胎成纤维细胞 (HEL)的影响。结果 :两种药物均可抑制HCMV在HEL中的增殖 ,但中药能明显抑制 p38mRNA的水平 ,并使其磷酸化底物 pRb蛋白表达降低 ,而GCV无此作用。 结论

Objective: To explore the mechanism of Jinye Baidu Granule (JYBDG) in treating human cytomegalovirus (HCMV) infection by studying its inhibitory effect on mitosin activating protein kinase (MAPK) pathway with HCMV infection. Methods: Expressions of p38 and pRb were investigated by means of in situ hybridization and immunohistochemistry before and after treated by JYBDG or Ganciclovir, and the development of cytopathic effect (CPE) was also observed. Results: Both JYBDG and Ganciclovir could inhibit HCMV proliferation in HEL, but JYBDG showed obvious inhibition on p38mRNA level and lower the pRb protein expression, while Ganciclovir didn′t show. Conclusion: JYBDG could inhibit the gene expression and duplication of HCMV through regulating MAPK pathway.