肠上皮下肌成纤维细胞与溃疡性结肠炎关系的初步探讨

A study of the relationship between intestinal subepithelial myofibroblasts and ulcerative colitis

目的 肠上皮下肌成纤维细胞 (ISEMF)是位于胃肠道黏膜上皮下的肌成纤维细胞 ,可分泌细胞因子、前列腺素、生长因子及细胞外基质蛋白 ,目前认为在炎症、组织修复、纤维化、肿瘤形成等方面起重要作用。通过建立肠镜活检组织标本检测ISEMF的方法 ,对ISEMF与溃疡性结肠炎 (溃结 )的关系进行初步探讨。方法 1 5例溃结患者和 2 0例对照者肠镜活检标本 ,应用免疫组化方法 ,采用鼠抗人α 平滑肌肌动蛋白单克隆抗体检测肠黏膜中ISEMF。结果 ISEMF位于结肠黏膜上皮细胞基底部 ,为椭圆形或舟状 ,正常对照组每高倍视野ISEMF为 56 .3± 6 .9,溃结组为 71 .3± 9.1 ,溃结组ISEMF增多 ,差异有显著性 (P <0 .0 0 1 )。结论 溃结组ISEMF有增多的趋势 。

Objective Intestinal subepithelial myofibroblasts(ISEMFs) exist throughout the gastrointestinal tract. Due to their ability to secrete cytokines, prostaglandins, growth factors, and extracellular matrix proteins, they are thought to play critical roles in inflammation, repair, fibrosis and neoplasia. The goal of this study was to investigate the relationship between ISEMFs and ulcerative colitis by establishing the method of ISEMFs detection from the biopsy specimens. Methods ISEMFs in colon biopsy specimens (15 from active ulcerative colitis patients and 20 from controls) were detected by immunohistochemistry with smooth muscle actin monoclonal antibody. Results ISEMFs existed in a subepithelial location of colon, were located in the basement of epithelial, and were oval or scaphoid in appearance. ISEMFs in active ulcerative colitis (71.3±9.1) were more than those in controls (56.3±6.9) per field of high power ( P <0.001). Conclusions ISEMFs in colonic mucosa of ulcerative colitis tend to up regulate the function of ISEMFs, and thus may improve the prognosis of ulcerative colitis.